甲基胆蒽转化的小鼠BALB/3T3中前列腺素合成酶活性升高。
Elevated prostaglandins synthetase activity in methylcholanthrene-transformed mouse BALB/3T3.
作者信息
Hong S L, Wheless C M, Levine L
出版信息
Prostaglandins. 1977 Feb;13(2):271-9. doi: 10.1016/0090-6980(77)90008-9.
Cell lines transformed from 3T3 spontaneously, by radiation, or by treatment with chemical carcinogens, polyoma and SV40 virus produce up to 5 times more prostaglandins than their untransformed parent line. Several aspects of prostaglandin biosynthesis by MC5-5 and 3T3 were compared. When stimulated by serum, bradykinin, or thrombin, MC5-5 cells labeled with radioactive arachidonic acid in their cellular lipids, these higher levels were shown not to be due to increased availability of the prostaglandin precursor, arachidonic acid. Prostaglandin synthetase activity in microsomal fractions prepared from MC5-5 was 6 times higher than that of microsomes of untransformed cells. The increased prostaglandin levels produced by transformed cells therefore appear to be the result of elevated prostaglandin synthetase activity.
通过辐射、化学致癌物、多瘤病毒和SV40病毒自发转化的3T3细胞系产生的前列腺素比未转化的亲代细胞系多5倍。对MC5-5和3T3细胞合成前列腺素的几个方面进行了比较。当受到血清、缓激肽或凝血酶刺激时，MC5-5细胞在其细胞脂质中被放射性花生四烯酸标记，结果表明这些较高水平并非由于前列腺素前体花生四烯酸的可用性增加。从MC5-5制备的微粒体部分中的前列腺素合成酶活性比未转化细胞的微粒体高6倍。因此，转化细胞产生的前列腺素水平升高似乎是前列腺素合成酶活性升高的结果。
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