D22S683和D6S477基因座上两个高变短串联重复序列的序列变异

Sequence variation of two hypervariable short tandem repeats at the D22S683 and D6S477 loci.

作者信息

Barral S, Lareu M V, Salas A, Carracedo A

机构信息

Institute of Legal Medicine, Faculty of Medicine, Santiago de Compostela, Spain.

出版信息

Int J Legal Med. 2000;113(3):146-9. doi: 10.1007/s004140050286.

DOI:10.1007/s004140050286

PMID:10876985

Abstract

For two short tandem repeats at the D22S683 and D6S477 loci, 30 and 22 selected alleles, respectively were sequenced. A total of 20 different alleles were found for the D22S683 locus and 12 alleles for the D6S477 locus. In both systems the alleles were designated according to the total number of repeats. D22S683 is a hypervariable STR consisting of blocks of (TATC) repeats with a basic sequence structure (TATATC)n (TATC)n (ATC)0-1 (TATC)n. The D6S477 locus consists of blocks of (TCTA) repeats with a basic sequence structure (TCTA)n (TA)1 (TCTA)0-2 (TA)0-1 (TCTA)n. Population data showed a heterozygosity of 0.89 for D22S683 and 0.75 for D6S477. These STRs are promising markers for forensic genetics as they are robust and can be easily included in multiplexes.

摘要

对于位于D22S683和D6S477基因座的两个短串联重复序列，分别对30个和22个选定的等位基因进行了测序。在D22S683基因座共发现20个不同的等位基因，在D6S477基因座发现12个等位基因。在这两个系统中，等位基因均根据重复总数进行命名。D22S683是一个高变STR，由（TATC）重复序列块组成，其基本序列结构为（TATATC）n（TATC）n（ATC）0-1（TATC）n。D6S477基因座由（TCTA）重复序列块组成，其基本序列结构为（TCTA）n（TA）1（TCTA）0-2（TA）0-1（TCTA）n。群体数据显示，D22S683的杂合度为0.89，D6S477的杂合度为0.75。这些STR是法医遗传学中有前景的标记物，因为它们稳定性好，且能很容易地纳入多重检测中。