Murrell A, Campbell N J, Barker S C
Department of Microbiology and Parasitology, The University of Queensland, Brisbane, Queensland, 4072, Australia.
Mol Phylogenet Evol. 2000 Jul;16(1):1-7. doi: 10.1006/mpev.2000.0762.
We inferred the phylogeny of 21 species and subspecies of ticks from the subfamilies Rhipicephalinae and Hyalomminae using cytochrome c oxidase subunit I (COI) and 12S rRNA mitochondrial gene sequences. Two members of the subfamily Haemaphysalinae were used for outgroup reference. The largest rhipicephaline genus, Rhipicephalus, was represented by ticks from six of the species groups, the second largest genus, Dermacentor, by species from two of three of its subgenera, and the genus Boophilus by 3 of its 5 species. We analyzed the 12S and COI sequences separately and together; statistically significant incongruence between the 12S rDNA and the COI sequences was not detected in the combined dataset using the incongruence length difference test. The combined dataset provided greater phylogenetic resolution than the individual datasets, and although the 12S rDNA data had only 25% of the parsimony-informative characters, it provided half of the total partitioned Bremer support for the combined dataset. We present the first hypothesis of phylogenetic relationships among some species groups of Rhipicephalus but our most controversial result was that the genus Rhipicephalus is apparently paraphyletic, unless species of Boophilus are included in it. The species of Rhipicephalus most closely related to Boophilus spp. were from the R. pravus and R. evertsi species groups, which may implicate an African origin for this important group of ticks.
我们利用细胞色素c氧化酶亚基I（COI）和12S rRNA线粒体基因序列，推断了璃眼蜱亚科和硬蜱亚科21种蜱虫及其亚种的系统发育关系。血蜱亚科的两个成员被用作外群参考。最大的硬蜱属，即硬蜱属，由来自六个物种组的蜱虫代表；第二大属，即革蜱属，由其三个亚属中的两个亚属的物种代表；而牛蜱属则由其五个物种中的三个物种代表。我们分别和共同分析了12S和COI序列；使用不一致长度差异检验，在合并数据集中未检测到12S rDNA和COI序列之间具有统计学意义的不一致。合并数据集比单个数据集提供了更高的系统发育分辨率，尽管12S rDNA数据只有25%的简约信息特征，但它为合并数据集提供了总划分布雷默支持的一半。我们提出了硬蜱属一些物种组之间系统发育关系的首个假设，但我们最具争议的结果是，硬蜱属显然是并系的，除非将牛蜱属的物种包括在内。与牛蜱属物种关系最密切的硬蜱属物种来自草原硬蜱和埃氏硬蜱物种组，这可能意味着这一重要蜱虫群体起源于非洲。
