霍乱斑点试验评估：一种用于快速检测霍乱抗原的色谱免疫分析法。
Evaluation of the cholera spot test: a chromatographic immunoassay for the rapid detection of cholera antigen.
作者信息
Rohani M Y, Hasnidah D, Ong K H
机构信息
Bacteriology Division, Institute for Medical Research, Kuala Lumpur, Malaysia.
出版信息
Malays J Pathol. 1998 Jun;20(1):31-3.
A chromatographic immunoassay cholera antigen detection kit, the Cholera Spot test, was evaluated. The test was found to be specific with a sensitivity of 10(6) cfu/ml for the direct detection of V. cholerae in simulated stool specimens and 10 cfu/ml in simulated cotton-tipped swab specimens after overnight incubation in alkaline peptone water. This enables early recognition of cholera cases and their contacts so that prevention and control measures can be promptly instituted.
对一种色谱免疫测定霍乱抗原检测试剂盒——霍乱斑点试验进行了评估。结果发现，该检测方法具有特异性，对于模拟粪便标本中霍乱弧菌的直接检测，灵敏度为10⁶ cfu/ml；在碱性蛋白胨水中过夜培养后，对模拟棉拭子标本的检测灵敏度为10 cfu/ml。这能够早期识别霍乱病例及其接触者，从而迅速采取预防和控制措施。
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