基于单克隆抗体的试剂盒Bengal SMART用于粪便样本中霍乱弧菌O139（同义词Bengal）快速检测的评估。
Evaluation of the monoclonal antibody-based kit Bengal SMART for rapid detection of Vibrio cholerae O139 synonym Bengal in stool samples.
作者信息
Qadri F, Hasan J A, Hossain J, Chowdhury A, Begum Y A, Azim T, Loomis L, Sack R B, Albert M J
机构信息
International Centre for Diarrhoeal Disease Research, Bangladesh, Dhaka.
出版信息
J Clin Microbiol. 1995 Mar;33(3):732-4. doi: 10.1128/jcm.33.3.732-734.1995.
Abstract
A monoclonal antibody-based test, Bengal SMART, was developed for rapid detection of Vibrio cholerae O139 synonym Bengal directly from stool specimens. The test, which takes about 15 min to complete, was used to screen 189 diarrheal stool specimens. The results were compared with those of a monoclonal antibody-based coagglutination test (COAT) and the conventional culture methods used as the "gold standard" for detection of V. cholerae O139. The Bengal SMART test showed a sensitivity of 100% and a specificity of 97% in comparison with the gold standard. It also fared better than COAT, which had a sensitivity of 96% for rapid detection of V. cholerae O139 synonym Bengal. These results show that Bengal SMART is suitable for use in field settings for rapid diagnosis of cholera caused by V. cholerae O139.
摘要
一种基于单克隆抗体的检测方法——孟加拉智能检测法（Bengal SMART）被开发出来，用于直接从粪便标本中快速检测霍乱弧菌O139（同义词孟加拉型）。该检测方法大约需要15分钟完成，用于筛查189份腹泻粪便标本。将结果与基于单克隆抗体的协同凝集试验（COAT）以及用作检测霍乱弧菌O139“金标准”的传统培养方法的结果进行比较。与金标准相比，孟加拉智能检测法的灵敏度为100%，特异性为97%。它的表现也优于COAT，COAT对快速检测霍乱弧菌O139同义词孟加拉型的灵敏度为96%。这些结果表明，孟加拉智能检测法适用于现场快速诊断由霍乱弧菌O139引起的霍乱。
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本文引用的文献
1
A novel kit for rapid detection of Vibrio cholerae O1.一种用于快速检测霍乱弧菌O1型的新型试剂盒。
J Clin Microbiol. 1994 Jan;32(1):249-52. doi: 10.1128/jcm.32.1.249-252.1994.
2
Vibrio cholerae O139 Bengal in Bangkok.曼谷的霍乱弧菌O139孟加拉型
Lancet. 1993 Aug 14;342(8868):430-1. doi: 10.1016/0140-6736(93)92841-g.
3
Vibrio cholerae non-O1--the eighth pandemic?非O1群霍乱弧菌——第八次大流行？
Lancet. 1993 Aug 14;342(8868):382-3. doi: 10.1016/0140-6736(93)92806-5.
4
Large outbreak of clinical cholera due to Vibrio cholerae non-O1 in Bangladesh.孟加拉国因非O1群霍乱弧菌引发的临床霍乱大规模暴发。
Lancet. 1993 Mar 13;341(8846):704. doi: 10.1016/0140-6736(93)90481-u.
5
Emergence of novel strain of Vibrio cholerae with epidemic potential in southern and eastern India.印度南部和东部出现具有流行潜力的新型霍乱弧菌菌株。
Lancet. 1993 Mar 13;341(8846):703-4. doi: 10.1016/0140-6736(93)90480-5.
6
Development and evaluation of rapid monoclonal antibody-based coagglutination test for direct detection of Vibrio cholerae O139 synonym Bengal in stool samples.基于单克隆抗体的快速协同凝集试验用于粪便样本中霍乱弧菌O139（同义词孟加拉型）直接检测的开发与评估
J Clin Microbiol. 1994 Jun;32(6):1589-90. doi: 10.1128/jcm.32.6.1589-1590.1994.
7
Vibrio cholerae O139 in Karachi, Pakistan.巴基斯坦卡拉奇的霍乱弧菌O139
Lancet. 1993 Dec 4;342(8884):1422-3. doi: 10.1016/0140-6736(93)92780-w.
8
Production, characterization, and application of monoclonal antibodies to Vibrio cholerae O139 synonym Bengal.霍乱弧菌O139（同义词孟加拉型）单克隆抗体的制备、特性鉴定及应用
Clin Diagn Lab Immunol. 1994 Jan;1(1):51-4. doi: 10.1128/cdli.1.1.51-54.1994.
9
Enumeration of Vibrio cholerae O1 in Bangladesh waters by fluorescent-antibody direct viable count.通过荧光抗体直接活菌计数法对孟加拉国水域中的霍乱弧菌O1进行计数。
Appl Environ Microbiol. 1987 Dec;53(12):2862-5. doi: 10.1128/aem.53.12.2862-2865.1987.
10
Polymerase chain reaction for detection of the cholera enterotoxin operon of Vibrio cholerae.用于检测霍乱弧菌霍乱肠毒素操纵子的聚合酶链反应。
J Clin Microbiol. 1991 Nov;29(11):2517-21. doi: 10.1128/jcm.29.11.2517-2521.1991.
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