Hansen W R, Nashold S W, Docherty D E, Brown S E, Knudson D L
U.S. Geological Survey, Biological Resources Division, National Wildlife Health Center, Madison, WI 53711, USA.
Avian Dis. 2000 Apr-Jun;44(2):266-74.
A recently developed polymerase chain reaction (PCR) assay was used for diagnosis of duck plague in waterfowl tissues from past and current cases of waterfowl mortality and to identify duck plague virus in combined cloacal/oral-pharyngeal swab samples from healthy mallards (Anas platyrhynchos) after a disease outbreak. The PCR was able to detect viral DNA from all the individual or pooled tissues assayed from 10 waterfowl, including liver and spleen samples from three Muscovy ducks (Cairina moschata domesticus) that did not yield virus isolates. The strong staining intensity of the PCR products from the waterfowl tissues indicated that large amounts of virus were present, even when virus was not isolated. Duck plague DNA was also detected in a cloacal swab sample from a wood duck (Aix sponsa) carcass submitted for diagnosis. The PCR assay identified duck plague DNA in 13 swab samples that produced virus isolates from carrier mallards sampled in 1981 after a duck plague die-off. The duck plague PCR clearly demonstrated the ability to quickly diagnose duck plague in suspect mortality cases and to detect virus shed by carrier waterfowl.
一种最近开发的聚合酶链反应（PCR）检测方法被用于诊断过去和当前水禽死亡病例的水禽组织中的鸭瘟，并在疾病爆发后从健康绿头鸭（Anas platyrhynchos）的泄殖腔/口咽联合拭子样本中鉴定鸭瘟病毒。PCR能够从10只水禽检测的所有单个或混合组织中检测到病毒DNA，包括来自三只未分离出病毒株的番鸭（Cairina moschata domesticus）的肝脏和脾脏样本。水禽组织PCR产物的强染色强度表明即使未分离出病毒，也存在大量病毒。在提交诊断的一只林鸳鸯（Aix sponsa）尸体的泄殖腔拭子样本中也检测到了鸭瘟DNA。PCR检测在1981年鸭瘟死亡事件后从带毒绿头鸭采集的13份拭子样本中鉴定出了鸭瘟DNA，这些样本分离出了病毒株。鸭瘟PCR清楚地证明了其能够在疑似死亡病例中快速诊断鸭瘟，并检测带毒水禽排出的病毒。
