衣藻的Nac2基因编码一种参与psbD mRNA稳定性的叶绿体类TPR蛋白。
The Nac2 gene of Chlamydomonas encodes a chloroplast TPR-like protein involved in psbD mRNA stability.
作者信息
Boudreau E, Nickelsen J, Lemaire S D, Ossenbühl F, Rochaix J D
机构信息
Departments of Molecular Biology and Plant Biology, University of Geneva, 30 Quai Ernest-Ansermet, 1211 Geneva 4, Switzerland.
出版信息
EMBO J. 2000 Jul 3;19(13):3366-76. doi: 10.1093/emboj/19.13.3366.
Abstract
The psbD mRNA, which encodes the D2 reaction center polypeptide of photosystem II, is one of the most abundant chloroplast mRNAs. We have used genomic complementation to isolate the nuclear Nac2 gene, which is required for the stable accumulation of the psbD mRNA in Chlamydomonas reinhardtii. Nac2 encodes a hydrophilic polypeptide of 1385 amino acids with nine tetratricopeptide-like repeats (TPRs) in its C-terminal half. Cell fractionation studies indicate that the Nac2 protein is localized in the stromal compartment of the chloroplast. It is part of a high molecular weight complex that is associated with non-polysomal RNA. Change of a conserved alanine residue of the fourth TPR motif by site-directed mutagenesis leads to aggregation of Nac2 protein and completely abrogates its function, indicating that this TPR is important for proper folding of the protein and for psbD mRNA stability, processing and/or translation.
摘要
编码光系统II的D2反应中心多肽的psbD mRNA是叶绿体中含量最丰富的mRNA之一。我们利用基因组互补技术分离出了莱茵衣藻中psbD mRNA稳定积累所必需的核Nac2基因。Nac2编码一个由1385个氨基酸组成的亲水性多肽，其C端一半有九个四肽重复序列（TPR）。细胞分级分离研究表明，Nac2蛋白定位于叶绿体的基质区室。它是与非多聚体RNA相关的高分子量复合物的一部分。通过定点诱变改变第四个TPR基序中一个保守的丙氨酸残基会导致Nac2蛋白聚集并完全丧失其功能，这表明该TPR对于蛋白质的正确折叠以及psbD mRNA的稳定性、加工和/或翻译很重要。
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