Song Y, Cao G M, Chen Z, Li J L
Department of Microbiology, China Agricultural University, Beijing.
Sheng Wu Gong Cheng Xue Bao. 2000 Jan;16(1):31-5.
Three types of colony, powdery gray, white and bald, were isolated from Streptomyces avermitilis ATCC31272. Among them, only the powdery grey one produces avermectins. Sa-76 strain was selected from the powdery grey strain by mutation with high energy electric flow, and its avermectins titer attainde 100 micrograms/mL in shaking flask. Avermectin B1 was extracted and purified from the mycelia of Sa-76, and identified by UV, IR, 1H-NMR, 13C-NMR and mass spectra. After Sa-76 strain was treated twice with NTG, a strain named Sa-76-8 was selected with avermectins titer over 2000 micrograms/mL. The Sa-76-8 strain was treated with NTG once again, and a high avermectins producing strain named as Sa-76-9 with avermectins titer up to 3500-4000 micrograms/mL was selected.
从阿维链霉菌ATCC31272中分离出三种菌落类型，即粉状灰色、白色和光秃型。其中，只有粉状灰色菌落产生阿维菌素。通过高能电流诱变从粉状灰色菌株中筛选出Sa - 76菌株，其在摇瓶中的阿维菌素效价达到100微克/毫升。从Sa - 76的菌丝体中提取并纯化阿维菌素B1，并通过紫外光谱、红外光谱、1H - 核磁共振、13C - 核磁共振和质谱进行鉴定。用亚硝基胍对Sa - 76菌株处理两次后，筛选出阿维菌素效价超过2000微克/毫升的菌株Sa - 76 - 8。再次用亚硝基胍处理Sa - 76 - 8菌株，筛选出阿维菌素高产菌株Sa - 76 - 9，其阿维菌素效价高达3500 - 4000微克/毫升。
