Almekinder J, Manda W, Soko D, Lan Y, Hoover D R, Semba R D
Johns Hopkins Medical Institutions, Johns Hopkins University, Baltimore, Maryland, USA.
Scand J Clin Lab Invest. 2000 May;60(3):199-203. doi: 10.1080/003655100750044848.
Plasma retinol-binding protein (RBP) concentrations have been suggested as surrogate indicators for plasma retinol concentrations in the assessment of vitamin A status in less technologically developed settings. Plasma RBP was measured by radial immunodiffusion and plasma retinol by high performance liquid chromatography in a cross-sectional study of 900 pregnant women at the Queen Elizabeth Central Hospital, Blantyre, Malawi. The Spearman correlation coefficient between plasma RBP and retinol concentrations was 0.95 (p<0.0001). By linear regression, 0.70 micromol l(-1) retinol was equivalent to 21.1 mg l(-1) RBP. With these cut-off points for defining vitamin A deficiency, there was high concordance between categorical descriptions of deficiency using retinol and RBP by chi-square analysis (p<0.001). Measurement of plasma RBP by radial immunodiffusion is simple, inexpensive, and does not require expensive instrumentation. Plasma RBP concentrations measured by radial immunodiffusion are highly correlated with plasma retinol and can be used as a simple surrogate measure for vitamin A concentrations in large field studies.
在技术欠发达地区评估维生素A状况时，血浆视黄醇结合蛋白（RBP）浓度被提议作为血浆视黄醇浓度的替代指标。在马拉维布兰太尔伊丽莎白女王中央医院对900名孕妇进行的一项横断面研究中，采用放射免疫扩散法测定血浆RBP，采用高效液相色谱法测定血浆视黄醇。血浆RBP与视黄醇浓度之间的Spearman相关系数为0.95（p<0.0001）。通过线性回归分析，0.70微摩尔/升视黄醇相当于21.1毫克/升RBP。利用这些界定维生素A缺乏的临界值，通过卡方分析发现，使用视黄醇和RBP对缺乏状况进行分类描述时具有高度一致性（p<0.001）。采用放射免疫扩散法测定血浆RBP简单、成本低，且无需昂贵的仪器设备。通过放射免疫扩散法测定的血浆RBP浓度与血浆视黄醇高度相关，可在大型现场研究中用作维生素A浓度的简单替代指标。
