Petersen I, Hidalgo A, Petersen S, Schlüns K, Schewe C, Pacyna-Gengelbach M, Goeze A, Krebber B, Knösel T, Kaufmann O, Szymas J, von Deimling A
Institute of Pathology, Charité - Campus Mitte, Berlin, FRG.
Brain Pathol. 2000 Jul;10(3):395-401. doi: 10.1111/j.1750-3639.2000.tb00271.x.
Metastases account for approximately 50% of the malignant tumors in the brain. In order to identify structural alterations that are associated with tumor dissemination into the central nervous system we used Comparative Genomic Hybridization (CGH) to investigate 42 brain metastases and 3 primary tumors of 40 patients. The metastases originated from lung cancer (14 cases), melanomas (7), carcinomas of breast (5), colon (5), kidney (5), adrenal gland (1) and thyroid (1). In addition, tumors of initially unknown primaries were assessed in 3 cases. The highest incidence of DNA gains were observed for the chromosomal regions 1q23, 8q24, 17q24-q25, 20q13 (>80% of cases) followed by the gain on 7p12 (77%). DNA losses were slightly less frequent with 4q22, 4q26, 5q21, 9p21 being affected in at least 70% of the cases followed by deletions at 17p12, 4q32q34, 10q21, 10q23-q24 and 18q21-q22 in 67.5% of cases. Two unusual narrow regional peaks were observed for the gain on 17q24-q25 and loss on 17p12. The incidence at individual loci can be viewed at our CGH online tumor database at http:// amba.charite.de/cgh/. The metastases of each tumor type showed a recurrent pattern of changes. In those cases with primary tumor and metastases available, the CGH pattern exhibited a high degree of conformity. In conclusion, our data suggests that specific genetic lesions are associated with tumor dissemination into the nervous system and that CGH analysis may be a useful supplementary tool for classification of metastases with unknown origin.
转移瘤约占脑恶性肿瘤的50%。为了确定与肿瘤扩散至中枢神经系统相关的结构改变，我们采用比较基因组杂交（CGH）技术对40例患者的42个脑转移瘤和3个原发性肿瘤进行了研究。转移瘤起源于肺癌（14例）、黑色素瘤（7例）、乳腺癌（5例）、结肠癌（5例）、肾癌（5例）、肾上腺癌（1例）和甲状腺癌（1例）。此外，3例患者的原发肿瘤最初不明。在染色体区域1q23、8q24、17q24 - q25、20q13观察到DNA增益的发生率最高（>80%的病例），其次是7p12的增益（77%）。DNA缺失的频率略低，至少70%的病例中4q22、4q26、5q21、9p21受到影响，其次是17p12、4q32q34、10q21、10q23 - q24和18q21 - q22的缺失，发生率为67.5%。在17q24 - q25的增益和17p12的缺失中观察到两个异常狭窄的区域峰。各个位点的发生率可在我们的CGH在线肿瘤数据库http://amba.charite.de/cgh/中查看。每种肿瘤类型的转移瘤都显示出一种反复出现的变化模式。在有原发性肿瘤和转移瘤的病例中，CGH模式表现出高度的一致性。总之，我们的数据表明特定的基因损伤与肿瘤扩散至神经系统相关，并且CGH分析可能是一种有用的辅助工具，用于对不明来源的转移瘤进行分类。
