[家蚕RAPD分子连锁图谱的构建]
[Construction of silkworm RAPD molecular linkage map].
作者信息
Li B, Lu C, Zhou Z Y, Xiang Z H
机构信息
Key Sericultural Laboratory of Agricultural Ministry, Southwest Agricultural University, Chongqing, China.
出版信息
Yi Chuan Xue Bao. 2000;27(2):127-32.
In this research, a RAPD linkage map of Bombyx mori was constructed with Dazao/C108 and their F2 generation. The map consists of 182 RAPD loci, of which 103 loci come from Dazao and from the first 23 linkage groups and the other 79 loci come from C108 and form the second 16 linkage groups. This map covered a total genetic distance of over 1,148.3 cM (centimorgan). It could be integrated with the SADF map of the same materials constructed in our laboratory and the corresponding RFLP linkage map.
本研究利用大造/C108及其F2代构建了家蚕的RAPD连锁图谱。该图谱由182个RAPD位点组成，其中103个位点来自大造，分布在前23个连锁群中，另外79个位点来自C108，形成了另外16个连锁群。该图谱覆盖的总遗传距离超过1148.3厘摩（cM）。它可以与我们实验室构建的相同材料的SADF图谱以及相应的RFLP连锁图谱整合。
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