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[大豆遗传图谱的构建与分析]

[Construction and analysis of soybean genetic map].

作者信息

Liu F, Zhuang B C, Zhang J S, Chen S Y

机构信息

Laboratory of Plant Biotechnology, Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China.

出版信息

Yi Chuan Xue Bao. 2000;27(11):1018-26.

Abstract

Molecular linkage maps provide a powerful tool for the analysis of plant genome structure and function including the detection of quantitative trait loci (QTL). The molecular linkage map of soybean was constructed using an 88-individual RIL population derived from a cross between cultivated Changnong 4 and semi-wild Xinmin 6. The map contains 22 linkage groups and 240 markers, including 100 RFLP, 33 SSR, 62 RAPD, 42 AFLP, 1 SCAR and 2 morphological markers. It covers approximately 3,713.5 cM of soybean genome. Analysis of 72 RFLP markers showed that 16 of them revealed two or more independent loci and it suggested that there is an extensive homologous region in soybean genome. This map compared very well with the other published soybean maps.

摘要

分子连锁图谱为分析植物基因组结构和功能提供了一个强大的工具,包括检测数量性状基因座(QTL)。大豆分子连锁图谱是利用一个由栽培品种长农4号和半野生品种新民6号杂交产生的含88个单株的重组自交系(RIL)群体构建的。该图谱包含22个连锁群和240个标记,包括100个RFLP标记、33个SSR标记、62个RAPD标记、42个AFLP标记、1个SCAR标记和2个形态学标记。它覆盖了大豆基因组约3713.5厘摩。对72个RFLP标记的分析表明,其中16个标记显示出两个或更多独立的基因座,这表明大豆基因组中存在广泛的同源区域。该图谱与其他已发表的大豆图谱相比非常吻合。

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