Parpinello G P, Versari A
Dipartimento di Biotecnologie Agrarie ed Ambientali, Università di Ancona, Italy.
J Chromatogr Sci. 2000 Jun;38(6):259-61. doi: 10.1093/chromsci/38.6.259.
A reliable high-performance liquid chromatography-refractive index detection method for the simultaneous analysis of glucose, methanol, and glycerol in a bioprocess fermentation media using direct injection is presented. The validation studies show a satisfactory selectivity, linearity, accuracy, and recovery of the method. The lowest concentration detectable for glucose and methanol is 3.5 and 6.7 mg/100 mL, respectively. This method could be an attractive choice for the analysis of these compounds not only in fermentation media but also in biomedical and environmental samples.
提出了一种可靠的高效液相色谱-折光指数检测方法，用于直接进样同时分析生物过程发酵培养基中的葡萄糖、甲醇和甘油。验证研究表明该方法具有令人满意的选择性、线性、准确性和回收率。葡萄糖和甲醇的最低检测浓度分别为3.5和6.7 mg/100 mL。该方法不仅对于发酵培养基中这些化合物的分析，而且对于生物医学和环境样品中这些化合物的分析可能都是一个有吸引力的选择。
