酿酒酵母GAL7启动子上转录干扰与起始的平衡
Balancing transcriptional interference and initiation on the GAL7 promoter of Saccharomyces cerevisiae.
作者信息
Greger I H, Aranda A, Proudfoot N
机构信息
Sir William Dunn School of Pathology, South Parks Road, University of Oxford, Oxford OX1 3RE, United Kingdom.
出版信息
Proc Natl Acad Sci U S A. 2000 Jul 18;97(15):8415-20. doi: 10.1073/pnas.140217697.
Abstract
Transcriptional termination of the GAL10 gene in Saccharomyces cerevisiae depends on the efficiency of polyadenylation. Either cis mutations in the poly(A) signal or trans mutations of mRNA 3' end cleavage factors result in GAL10 read-through transcripts into the adjacent GAL7 gene and inactivation (occlusion) of the GAL7 promoter. Herein, we present a molecular explanation of this transcriptional interference phenomenon. In vivo footprinting data reveal that GAL7 promoter occlusion is associated with the displacement of Gal4p transcription factors from the promoter. Interestingly, overexpression of Gal4p restores promoter occupancy, activates GAL7 expression, and rescues growth on the otherwise toxic galactose substrate. Our data therefore demonstrate a precise balance between transcriptional interference and initiation.
摘要
酿酒酵母中GAL10基因的转录终止取决于聚腺苷酸化的效率。聚腺苷酸信号的顺式突变或mRNA 3'端切割因子的反式突变都会导致GAL10通读转录本进入相邻的GAL7基因，并使GAL7启动子失活（封闭）。在此，我们对这种转录干扰现象给出了分子层面的解释。体内足迹数据显示，GAL7启动子封闭与Gal4p转录因子从启动子上的位移有关。有趣的是，Gal4p的过表达可恢复启动子占据情况，激活GAL7表达，并挽救在原本有毒的半乳糖底物上的生长。因此，我们的数据证明了转录干扰与起始之间的精确平衡。
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