Murohashi T, Yoshida K
Acta Leprol. 1978 Oct-Dec(73):13-28.
Drug sensitivity was tested using liquid medium on three stains isolated from the subcutaneous nodules of L-type patients who have long been administered DDS alone. The results revealed that the first strain was resistant to DDS up to the concentration of 1.0 microgram/ml suggesting as if it were DDS dependent or enhanced strain, whereas the second strain was completely sensitive to DDS even at the lowest concentration of 0.01 microgram/ml suggesting possible inactivation of this drug in the host patient. The third strain was completely resistant to 0.1 microgram/ml, but sensitive to 1.0 microgram/ml of DDS, suggesting that the therapeutic effect can not be expected any more, when the strain becomes resistant to 0.1 microgram/ml of DDS. All of the three strains were sensitive to REP at the concentration of 0.01 microgram/ml, and the host patients of the former two strains showed rapid improvement of the clinical symptomes after REP administration. That the second strain was sensitive to INH at the concentratin of 0.01 microgram/ml suggested the availability of the combined use of INH in the chemotherapy of leprosy.
使用液体培养基对从长期单独服用氨苯砜的L型患者皮下结节中分离出的三株菌株进行了药敏试验。结果显示，第一株菌株对高达1.0微克/毫升浓度的氨苯砜耐药，似乎是氨苯砜依赖型或增强型菌株，而第二株菌株即使在最低浓度0.01微克/毫升时对氨苯砜也完全敏感，提示该药物在宿主患者体内可能失活。第三株菌株对0.1微克/毫升完全耐药，但对1.0微克/毫升的氨苯砜敏感，这表明当该菌株对0.1微克/毫升的氨苯砜耐药时，就不能再期待有治疗效果了。所有三株菌株对0.01微克/毫升浓度的利福平敏感，前两株菌株的宿主患者在服用利福平后临床症状迅速改善。第二株菌株对0.01微克/毫升浓度的异烟肼敏感，提示在麻风病化疗中联合使用异烟肼的可行性。
