Griffin J R, Straatsma B R, Kreiger A E
Am J Ophthalmol. 1975 Jan;79(1):25-38. doi: 10.1016/0002-9394(75)90452-3.
Transvitreal chorioretinal biopsy was performed in nine Dutch rabbits by introducing a biopsy instrument through a small eye wall incision, passing the instrument across the eye through the vitreous to the biopsy site, and by trephinating and removing from the eye a 1.6-mm diameter specimen of the posterior eye wall. Biopsy was carried out under stereotaxic control by using a micromanipulator especially designed for ophthalmic surgery. Hemostasis was achieved by circumferential mechanical pressure around the biopsy site. The eye wall defect was sealed by a preplaced explant and repaired by fibrovascular ingrowth from the surrounding choroid and episclera. The normal histologic features of the biopsy specimen were preserved, except for separation of the neurosensory retina from the pigmented epithelium. Complications of the procedure included hemorrhage from the incision site, transvitreal strand formation, and lens abrasion. In no instance, however, did these complications result in significant opacification of the optical media. The biopsy technique has potential use as a clinical procedure in the diagnosis of choroidal tumors and other selected disorders of the choroid and retina and as a technique in investigational animal studies.
通过在九只荷兰兔的眼壁上做一个小切口，将活检器械经此切口穿过眼球玻璃体到达活检部位，然后通过环钻并从眼球取出直径1.6毫米的眼后壁标本，进行经玻璃体脉络膜视网膜活检。活检是在立体定位控制下，使用专门为眼科手术设计的显微操作器进行的。通过在活检部位周围施加环形机械压力来实现止血。眼壁缺损由预先放置的植入物封闭，并通过周围脉络膜和巩膜的纤维血管长入进行修复。活检标本的正常组织学特征得以保留，但神经感觉视网膜与色素上皮分离除外。该手术的并发症包括切口部位出血、经玻璃体条索形成和晶状体擦伤。然而，这些并发症在任何情况下都未导致光学介质明显混浊。这种活检技术有潜力作为一种临床方法用于诊断脉络膜肿瘤及其他特定的脉络膜和视网膜疾病，也可作为一种技术用于动物研究。
