Benoy M J, Essy A K, Sreekumar B, Haridas M
School of Biosciences, Mahatma Gandhi University, Kottayam.
Life Sci. 2000;66(25):2433-9. doi: 10.1016/s0024-3205(00)80003-x.
Goat milk lactoperoxidase was purified using CM-Sephadex-C-50 ion exchange chromatography and Sephadex G-100 gel filtration. The purified protein was found to have high antifungal and antibacterial activity in a thiocyanate-H2O2 medium. The results are relevant enough to suggest the exploitation of LP-thiocyanate-H2O2 system as an effective agent against many of the disease causing organisms in plants and animals.
采用CM - 葡聚糖凝胶C - 50离子交换色谱法和葡聚糖凝胶G - 100凝胶过滤法对山羊乳中的乳过氧化物酶进行了纯化。结果发现，纯化后的蛋白质在硫氰酸盐 - H2O2介质中具有较高的抗真菌和抗菌活性。这些结果足以表明，利用乳过氧化物酶 - 硫氰酸盐 - H2O2体系作为一种有效试剂来对抗动植物中许多致病生物是可行的。
