Recht J, Martínez A, Torello S, Kolter R
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
J Bacteriol. 2000 Aug;182(15):4348-51. doi: 10.1128/JB.182.15.4348-4351.2000.
A screen for nonsliding mutants of Mycobacterium smegmatis yielded 20 mutants with transposon insertions in the mps gene, which is involved in glycopeptidolipid biosynthesis. One mutant had an insertion in a gene predicted to encode a membrane transport protein. All mutants lacked glycopeptidolipids and were unable to form biofilms on polyvinyl chloride.
耻垢分枝杆菌非滑动突变体的筛选产生了20个在mps基因中有转座子插入的突变体,该基因参与糖肽脂生物合成。一个突变体在一个预测编码膜转运蛋白的基因中有插入。所有突变体都缺乏糖肽脂,并且无法在聚氯乙烯上形成生物膜。