Berg R D, Savage D C
Infect Immun. 1975 Feb;11(2):320-9. doi: 10.1128/iai.11.2.320-329.1975.
Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous bacteria. PFC reacting with these bacterial antigens were not detected in infant SPF mice until 7 days after birth. Compared with nonvaccinated controls, SPF mice vaccinated parenterally with indigenous E. coli or Bacteroides produced a moderate increase in the numbers of specific PFC. Thus, the SPF mouse is capable of responding immunologically after vaccination with microbes indigenous to its intestinal tract. However, more PFC reacting with homologous vaccine antigens were detected after parenteral vaccination of SPF mice with nonindigenous E. coli O127:B8, E.coli O14, or B. fragilis than after parenteral vaccination with indigenous E. coli or Bacteroides. Gnotobiotic mice orally monoassociated with these nonindigenous bacteria exhibited greater immune responses to antigens of the bacteria used to monoassociation than did gnotobiotes monoassociated with the indigenous microbes. The results are consistent with the hypothesis that mice are more responsive immunologically to antigens of nonindigenous bacteria than they are to antigens of certain microbes indigenous to their gastrointestinal tracts.
从无特定病原体（SPF）小鼠的胃肠道中分离出本土大肠杆菌、拟杆菌和乳酸杆菌菌株。未接种疫苗的SPF小鼠脾脏中与这些本土细菌抗原发生反应的噬斑形成细胞（PFC）和玫瑰花结形成细胞数量较少。在出生后7天之前，未在新生SPF小鼠中检测到与这些细菌抗原发生反应的PFC。与未接种疫苗的对照组相比，经皮下接种本土大肠杆菌或拟杆菌的SPF小鼠产生的特异性PFC数量适度增加。因此，SPF小鼠在接种其肠道内的本土微生物疫苗后能够产生免疫反应。然而，与用本土大肠杆菌或拟杆菌进行皮下接种相比，用非本土大肠杆菌O127:B8、大肠杆菌O14或脆弱拟杆菌对SPF小鼠进行皮下接种后，检测到更多与同源疫苗抗原发生反应的PFC。与用本土微生物进行单关联的无菌动物相比，经口与这些非本土细菌进行单关联的无菌动物对用于单关联的细菌抗原表现出更强的免疫反应。这些结果与以下假设一致，即小鼠对非本土细菌抗原的免疫反应比对其胃肠道中某些本土微生物抗原的免疫反应更强。
