Mieczkowski T
Department of Criminology, The University of South Florida, St. Petersburg 33701, USA.
Life Sci. 2000 May 26;67(1):39-43. doi: 10.1016/s0024-3205(00)00601-9.
Psillakis et al. have reported on the concentration of carbamazepine recovered from hair samples collected from patients receiving this anti-seizure medication under medical supervision and determined that their was a high correlation between dose and quantitation of recovered analyte. The analyte was identified by two techniques, FPIA (Abbott TDx) and HPLC and the correlation was high for both procedures. In the literature on hair analysis some have suggested that analyte concentration in hair is critically dependent on hair color. In reporting their data Psillakis et al. reported the hair color of each patient but made no attempt to analyze their results in relation to color. This article performs a secondary analysis of the Psillakis et al. data in order to determine whether there is a hair color effect discernible in the recovery of carbamazepine from hair. Analysis of this data set for both the FPIA and HPLC by one-way analysis of variance fails to identify a color effect at p = .05. Weighting the data for per-patient dosage values fails to discern a color effect. Examination of all possible two-color comparisons also fails to identify a statistically significant effect for any subset of combinations. These data suggest that carbamazepine does not exhibit a color effect when using either FPIA or HPLC assay methods.
西拉基斯等人报告了从在医学监督下接受这种抗癫痫药物治疗的患者所采集的毛发样本中回收的卡马西平浓度，并确定回收分析物的剂量与定量之间存在高度相关性。该分析物通过两种技术进行鉴定，即荧光偏振免疫分析（雅培TDx）和高效液相色谱法，且两种方法的相关性都很高。在毛发分析的文献中，一些人认为毛发中的分析物浓度严重依赖于头发颜色。西拉基斯等人在报告他们的数据时报告了每位患者的头发颜色，但并未尝试根据颜色分析其结果。本文对西拉基斯等人的数据进行二次分析，以确定从毛发中回收卡马西平时是否存在可辨别的头发颜色效应。通过单因素方差分析对该数据集的荧光偏振免疫分析和高效液相色谱法进行分析，在p = 0.05时未发现颜色效应。根据每位患者的剂量值对数据进行加权，也未发现颜色效应。对所有可能的双色比较进行检查，也未发现任何组合子集具有统计学意义的效应。这些数据表明，使用荧光偏振免疫分析或高效液相色谱法测定时，卡马西平不会表现出颜色效应。
