Guan J, Gao C, Feng L, Sheng J
Department of Polymer Science and Engineering, Zhejiang University, Hangzhou, People's Republic of China.
J Biomater Sci Polym Ed. 2000;11(5):523-36. doi: 10.1163/156856200743841.
Cytocompatible polyurethane (PU) surface was prepared by photo-grafting 2-hydroxyethyl acrylate (HEA) onto the membrane surface. Graft polymerization was conducted by combining the use of the photo-oxidation and irradiation grafting. PU membrane was photo-oxidized to introduce the hydroperoxide groups onto the surface, then the membrane, immersed previously in monomer solution, was irradiated under UV light. The ATR-FTIR spectra, element spectroscopy for chemical analysis (ESCA), scanning electron microscopy (SEM) and water contact angle characterized the grafted copolymers and verified the occurrence of graft polymerization. The results showed that UV irradiation could realize the graft polymerization effectively and the grafting was confined within the surface layer. The grafted membrane showed minimal surface morphology. Human umbilical vein endothelial (HUVE) cells were seeded on the grafted surface. The performance of the surface in cell attachment and growth correlated with the oxygen content and mainly the carbonyl content on the surface. Cells were spread more extensively and grew faster on the surface with a higher oxygen content.
通过将丙烯酸羟乙酯（HEA）光接枝到膜表面制备了具有细胞相容性的聚氨酯（PU）表面。接枝聚合通过光氧化和辐照接枝相结合的方法进行。将PU膜进行光氧化以在其表面引入过氧化氢基团，然后将预先浸入单体溶液中的膜在紫外光下照射。通过衰减全反射傅里叶变换红外光谱（ATR-FTIR）、化学分析用元素光谱法（ESCA）、扫描电子显微镜（SEM）和水接触角对接枝共聚物进行表征，并验证接枝聚合的发生。结果表明，紫外辐照能够有效地实现接枝聚合，且接枝仅局限于表面层。接枝后的膜表面形态变化最小。将人脐静脉内皮（HUVE）细胞接种在接枝表面。该表面在细胞附着和生长方面的性能与表面的氧含量以及主要是羰基含量相关。在氧含量较高的表面上，细胞铺展更广泛且生长更快。
