Hsu S H, Chen W C
Department of Chemical Engineering, National Chung Hsing University, Taichung, Taiwan, Republic of China.
Biomaterials. 2000 Feb;21(4):359-67. doi: 10.1016/s0142-9612(99)00191-x.
Lactide-grafted polyurethanes were prepared by exposing the polyurethane films to argon plasma discharge, followed by grafting L-lactide onto the plasma-treated surface. The modified surfaces were characterized by measuring the static contact angle and by electron spectroscopy for chemical analysis (ESCA). The water contact angle of polyurethanes was decreased by L-lactide grafting, indicating hydrophilicity of the modified surface. Grafting also increased the O/C atomic ratio and C(C=O)/Ctotal percentage on the surfaces as detected by ESCA. The grafted surfaces showed enhanced attachment and growth in both 3T3 fibroblast and human umbilical vein endothelial cell culture tests. Platelet adhesion to the modified surfaces was also reduced in vitro. L-Lactide monomers grafted onto polyurethane substrates could therefore be useful in facilitating endothelial cell seeding process in small vascular graft applications.
通过将聚氨酯薄膜暴露于氩等离子体放电，随后将L-丙交酯接枝到经等离子体处理的表面上，制备了丙交酯接枝的聚氨酯。通过测量静态接触角和进行化学分析电子能谱（ESCA）对改性表面进行表征。L-丙交酯接枝降低了聚氨酯的水接触角，表明改性表面具有亲水性。ESCA检测结果还表明，接枝还增加了表面的O/C原子比和C(C=O)/C总百分比。在3T3成纤维细胞和人脐静脉内皮细胞培养试验中，接枝表面均显示出增强的细胞附着和生长。体外实验还表明，血小板在改性表面的黏附也有所减少。因此，接枝到聚氨酯基材上的L-丙交酯单体可用于促进小血管移植物应用中的内皮细胞接种过程。
