Marrack P C, Kappler J W
J Immunol. 1975 Mar;114(3):1116-25.
Evidence is presented for two types of helper T cells in the mouse specific for a protein antigen, keyhole limpet hemocyanin (KLH). The first cell is able to help B cells respond to the hapten, trinitrophenyl (TNP), when coupled to KLH presumably through the participation of KLH-specific T cell factors. The second helper cell when stimulated with KLH is able to help B cells respond to red blood cell antigens, but not TNP-KLH, through the production of a non-antigen-specific factor. Both types of helper cells were shown to be present in the spleens of KLH-primed mice, although they occurred with different frequencies. They were shown to be different T cells by their segregation from each other in vitro at limiting dilution.
有证据表明，小鼠体内存在两种针对蛋白质抗原——钥孔戚血蓝蛋白（KLH）的辅助性T细胞。第一种细胞能够在三硝基苯（TNP）与KLH偶联时，通过KLH特异性T细胞因子的参与，帮助B细胞对该半抗原产生反应。第二种辅助性细胞在用KLH刺激后，能够通过产生一种非抗原特异性因子，帮助B细胞对红细胞抗原产生反应，但不能帮助其对TNP-KLH产生反应。尽管这两种辅助性细胞出现的频率不同，但在经KLH免疫的小鼠脾脏中均有发现。通过在体外有限稀释条件下将它们彼此分离，证明它们是不同的T细胞。
