Harwell L, Kappler J W, Marrack P
J Immunol. 1976 May;116(5):1379-84.
T cell-containing lymphoid populations produce a nonantigen-specific mediator(s) (NSM) which can replace T cell helper function in vitro in the response of B cells to sheep red blood cells (SRBC), but not to the hapten-protein conjugate, trinitrophenyl-keyhole limpet hemocyanin, (TNP-KLH). NSM produced under three conditions: 1) stimulation of KLH-primed cells with KLH; 2) allogeneic stimulation of normal spleen cells; and 3) stimulation of normal spleen cells with Con A (but not PHA) are indistinguishable on the basis of their biologic activity and m.w., estimated as 30 to 40,000 daltons by G-200 chromatography. Production of NSM is dependent on the presence of T cells. The action of NSM on B cells responding to SRBC in the presence of 2-mercaptoethanol is unaffected by severe macrophage depletion. Extensive absorption of NSM with SRBC failed to remove its activity, confirming its nonantigen-specific nature.
含T细胞的淋巴细胞群体可产生一种非抗原特异性介质（NSM），在体外B细胞对绵羊红细胞（SRBC）的应答中，它能替代T细胞辅助功能，但对半抗原-蛋白质偶联物三硝基苯-钥孔血蓝蛋白（TNP-KLH）则不能。在以下三种条件下产生的NSM：1）用钥孔血蓝蛋白（KLH）刺激经KLH预致敏的细胞；2）对正常脾细胞进行同种异体刺激；3）用刀豆蛋白A（Con A）（而非植物血凝素PHA）刺激正常脾细胞，根据其生物学活性和分子量（经G-200层析估计为30000至40000道尔顿），这些NSM无法区分。NSM的产生依赖于T细胞的存在。在2-巯基乙醇存在的情况下，NSM对响应SRBC的B细胞的作用不受严重巨噬细胞耗竭的影响。用SRBC对NSM进行广泛吸附未能去除其活性，证实了其非抗原特异性的性质。
