Kaul P, Malla N, Kaur S, Mahajan R C, Ganguly N K
Department of Experimental Medicine, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Trans R Soc Trop Med Hyg. 2000 Mar-Apr;94(2):173-5. doi: 10.1016/s0035-9203(00)90264-5.
A purified 200-kDa antigenic fraction from Leishmania donovani axenic amastigotes was evaluated by ELISA for the detection of antibody response in visceral leishmaniasis (VL) patients, post kala-azar dermal leishmaniasis (PKDL) patients and controls, for the diagnosis of visceral leishmaniasis. A positive antibody response to the 200-kDa amastigote fraction and to Leishmania amastigote soluble antigen (LASA) was found in 29 (96.6%) and 30 ((100%) confirmed VL patients, respectively, by the use of ELISA. However, only 1 (10%) out of 10 PKDL patients had detectable antibody response to 200-kDa fraction while all the 10 (100%) PKDL patients exhibited an immune response to LASA. Therefore, use of the 200-kDa antigenic fraction for the detection of antibody response in an ELISA follow-up (post treatment) of VL patients may have prognostic significance, and it may also be useful for differentiating active VL and PKDL.
通过酶联免疫吸附测定（ELISA）对来自杜氏利什曼原虫体外无鞭毛体的纯化200 kDa抗原组分进行评估，以检测内脏利什曼病（VL）患者、黑热病后皮肤利什曼病（PKDL）患者及对照者体内的抗体反应，用于内脏利什曼病的诊断。通过ELISA检测发现，29例（96.6%）确诊的VL患者对200 kDa无鞭毛体组分呈阳性抗体反应，30例（100%）确诊的VL患者对利什曼原虫无鞭毛体可溶性抗原（LASA）呈阳性抗体反应。然而，10例PKDL患者中仅1例（10%）对200 kDa组分有可检测到的抗体反应，而所有10例（100%）PKDL患者均对LASA呈现免疫反应。因此，在VL患者的ELISA随访（治疗后）中使用200 kDa抗原组分检测抗体反应可能具有预后意义，也可能有助于区分活动性VL和PKDL。
