Ohkubo A, Tanaka Y, Ida M, Ohta H, Sakurabayashi I
KDK Corporation, Kyoto.
Rinsho Byori. 2000 Jun;48(6):533-9.
Using whole blood sample, we examined the correlation between concentration of granulocyte elastase (GEL) in granulocyte obtained by immunoassay and white blood cell counts. The correlation between concentration of GEL and granulocyte cell counts was also examined. The correlation coefficient between concentration of GEL and white blood cell counts was R = 0.87, and that between concentration of GEL and granulocyte cell counts was R = 0.91. The correlation coefficients of outpatients and patients with diseases accompanied by inflammation except for tumors were better than those of inpatients. The GEL concentration in granulocyte using whole blood sample well responded to the white blood cell counts against the tolerance for a short time, such as after operations. Especially on screening tests of diseases accompanied by inflammation and primary care, analysis of GEL in whole blood is not only useful for observation of inflammation and its progress but also suggests possibility of converting GEL to white blood cell counts or granulocyte. Furthermore, because it can be measured by easy-operative latex agglutination turbidimetric method, an easy measurement system can be built. Extended usage of the system as a rapid diagnostic tool on emergency tests in general clinics and hospitals are expected.
我们使用全血样本，通过免疫测定法检测了粒细胞中粒细胞弹性蛋白酶（GEL）浓度与白细胞计数之间的相关性。同时也检测了GEL浓度与粒细胞计数之间的相关性。GEL浓度与白细胞计数之间的相关系数为R = 0.87，GEL浓度与粒细胞计数之间的相关系数为R = 0.91。门诊患者以及除肿瘤外伴有炎症疾病患者的相关系数优于住院患者。使用全血样本检测粒细胞中的GEL浓度，对于诸如手术后短时间内的耐受性等情况，能很好地反映白细胞计数。特别是在伴有炎症疾病的筛查试验和初级保健中，全血中GEL的分析不仅有助于观察炎症及其进展，还提示了将GEL转换为白细胞计数或粒细胞计数的可能性。此外，由于它可以通过易于操作的乳胶凝集比浊法进行测量，因此可以建立一个简便的测量系统。预计该系统作为普通诊所和医院急诊检测的快速诊断工具会得到更广泛的应用。
