Till H, Metzger R, Mempel T, Boehm R, Joppich I
Department of Pediatric Surgery, University of Munich, Germany.
Pediatr Surg Int. 2000;16(4):293-6. doi: 10.1007/s003830050747.
The present study investigated the histologic maturation, proliferative capacity, and steroid production of fetal adrenal transplants (Tx) in adrenalectomized rats. A pair of fetal adrenal glands (18-20 days of gestation) was transplanted into the omentum of syngeneic Lewis rats (n = 45). Four weeks later, in 5 animals the grafts were excised for morphologic evaluation. Proliferation was investigated by immunohistochemical staining for KI-67 protein and quantified by the proliferation index (PI = positive cells/100 counts). All other hosts (Tx; n = 40) underwent bilateral adrenalectomy (AE) to induce Addisonian crisis. Postoperatively, survival and concentrations of potassium, sodium, aldosterone, and corticosterone were recorded for 6 months. These data were compared to controls (C=only AE; n = 30) and a sham group (S; n = 10). At the end of the study period all surviving hosts were killed for histologic examination of grafts. At 4 weeks post-Tx the adrenal grafts demonstrated a distinct zona glomerulosa and frequent proliferation with a PI of 0.084, comparable to normal control (0.092). Following AE survival was significantly prolonged in Tx (86% vs 12% of C, P < 0.05). Control animals developed severe hyponatremia and hyperkalemia, whereas in Tx only transient signs of Addisonian crisis were recorded. Levels of aldosterone dropped within 7 days in the Tx and C groups, but returned to normal for Tx within 8 weeks. Corticosterone levels of Tx animals fell to 25% within week, but steadily increased to 70% by the end of the study. At 6 months, grafts revealed a mature adrenocortical structure with little proliferative activity, which was comparable to controls. In a syngeneic rat model fetal adrenal transplants thus mature and proliferate to provide sufficient steroid production for adrenalectomized hosts.
本研究调查了肾上腺切除大鼠体内胎儿肾上腺移植（Tx）的组织学成熟度、增殖能力和类固醇生成情况。将一对胎儿肾上腺（妊娠18 - 20天）移植到同基因Lewis大鼠（n = 45）的大网膜中。四周后，对5只动物的移植物进行切除以进行形态学评估。通过免疫组织化学染色检测KI - 67蛋白来研究增殖情况，并通过增殖指数（PI = 阳性细胞数/100个计数）进行定量分析。所有其他宿主（Tx；n = 40）接受双侧肾上腺切除术（AE）以诱发艾迪生病危象。术后记录6个月的生存率以及钾、钠、醛固酮和皮质酮的浓度。将这些数据与对照组（C = 仅接受AE；n = 30）和假手术组（S；n = 10）进行比较。在研究期结束时，所有存活的宿主均被处死以对移植物进行组织学检查。Tx后4周，肾上腺移植物显示出明显的球状带且增殖频繁，PI为0.084，与正常对照组（0.092）相当。AE后，Tx组的生存率显著延长（86%对C组的12%，P < 0.05）。对照动物出现严重低钠血症和高钾血症，而在Tx组仅记录到短暂的艾迪生病危象迹象。Tx组和C组的醛固酮水平在7天内下降，但Tx组在8周内恢复正常。Tx组动物的皮质酮水平在一周内降至25%，但在研究结束时稳步升至70%。6个月时，移植物显示出成熟的肾上腺皮质结构且增殖活性较低，这与对照组相当。因此，在同基因大鼠模型中，胎儿肾上腺移植物能够成熟并增殖，为肾上腺切除的宿主提供足够的类固醇生成。
