Till H, Kellnar S t, Böhm R, Schwarz H P, Barretton G, Joppich I
Department of Pediatric Surgery, University of Munich, Germany.
J Pediatr Surg. 1997 Oct;32(10):1455-7. doi: 10.1016/s0022-3468(97)90561-3.
BACKGROUND/PURPOSE: The present study investigates a new laparoscopic technique for fetal adrenal transplantation in rats.
The procedure was successful in 9 of 10 cases (one hole in the omentum) with no postoperative complications. On examination 4 weeks postoperatively, all but one graft showed macroscopic integrity, vascular supply, and histological maturation to normal zonal differentiation. When bilateral adrenalectomy was performed in the recipient to assess endocrine competence of the fetal adrenal grafts, survival was prolonged and Addison crisis was prevented in the animals that underwent transplantation. Levels of aldosterone dropped within the first week after adrenalectomy, but recovered steadily. Analysis of corticosterone demonstrated that levels fell to 25% of sham operated rats in the first week, but then steadily climbed to 70%.
To the authors' knowledge this report presents the first study for laparoscopic transplantation of fetal tissues. Laparoscopic transplantation of fetal adrenal glands seemed feasible and successful in rats. The fetal adrenal transplants matured and served for a prolonged survival.
背景/目的：本研究探讨一种用于大鼠胎儿肾上腺移植的新腹腔镜技术。
10例中有9例手术成功（网膜有一处穿孔），术后无并发症。术后4周检查时，除1个移植物外，其余所有移植物均显示出宏观完整性、血管供应以及向正常区域分化的组织学成熟度。当对受体进行双侧肾上腺切除术以评估胎儿肾上腺移植物的内分泌功能时，接受移植的动物生存期延长且艾迪生病危象得到预防。肾上腺切除术后第一周醛固酮水平下降，但随后稳步恢复。皮质酮分析表明，术后第一周水平降至假手术大鼠的25%，但随后稳步攀升至70%。
据作者所知，本报告是胎儿组织腹腔镜移植的首次研究。胎儿肾上腺的腹腔镜移植在大鼠中似乎可行且成功。胎儿肾上腺移植物成熟并有助于延长生存期。
