Trammer A, Kellnar S, Welsch U, Schwarz H P
Kinderchirurgische Klinik im Dr. von Haunerschen Kinderspital, Ludwig-Maximilians-Universität München.
Eur J Pediatr Surg. 1994 Aug;4(4):249-52. doi: 10.1055/s-2008-1066113.
In a syngeneic rat model we investigated the feasibility of fetal adrenal gland transplantation into adult recipient animals. We determined the optimal site of graft implantation by morphological criteria. Transplantation into the omentum majus proved superior to transplantation underneath the fascia of the rectus abdominis muscle possibly due to superior access to vascular supply. We assessed transplant function by sequential determinations of serum corticosterone, aldosterone, sodium and body weight. We compared rats which received syngeneic fetal adrenal transplants and underwent excision of their own adrenal glands four weeks thereafter to control animals without adrenalectomy or transplantation and to adrenalectomized animals without prior transplantation. The total study period was 16 weeks. Whereas all adrenalectomized rats without prior transplantation died within 2 weeks, all the animals which had received fetal adrenal grafts survived. Their serum sodium remained within normal limits. Weight gain and serum corticosterone levels were decreased when compared to control animals. Aldosterone levels dropped intermittently but had normalized by 16 weeks. We conclude that fetal adrenal grafts may be able to substitute for the animals' own adrenal glands in the syngeneic model.
在同基因大鼠模型中，我们研究了将胎儿肾上腺移植到成年受体动物体内的可行性。我们通过形态学标准确定了移植物植入的最佳部位。事实证明，将肾上腺移植到大网膜中比移植到腹直肌筋膜下更具优势，这可能是因为大网膜更容易获得血管供应。我们通过连续测定血清皮质酮、醛固酮、钠和体重来评估移植功能。我们将接受同基因胎儿肾上腺移植并在四周后切除自身肾上腺的大鼠与未进行肾上腺切除术或移植的对照动物以及未预先移植的肾上腺切除动物进行了比较。整个研究期为16周。所有未预先移植的肾上腺切除大鼠均在2周内死亡，而所有接受胎儿肾上腺移植的动物均存活。它们的血清钠保持在正常范围内。与对照动物相比，体重增加和血清皮质酮水平降低。醛固酮水平间歇性下降，但在16周时恢复正常。我们得出结论，在同基因模型中，胎儿肾上腺移植物可能能够替代动物自身的肾上腺。
