Hernandez R, He H, Chen A C, Waghela S D, Wayne Ivie G, George J E, Gale Wagner G
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467, USA.
Insect Biochem Mol Biol. 2000 Oct;30(10):969-77. doi: 10.1016/s0965-1748(00)00069-2.
Two esterase cDNA sequences were obtained from susceptible and organophosphorus resistant strains of Boophilus microplus. Both sequences have a high degree of homology to carboxylesterase B. One gene has identical sequences in both strains and the other showed two point mutations. One mutation produces an amino acid substitution when the amino acid sequence is deduced, this mutation was detected in six different populations susceptible and resistant to insecticides, but a pyrethroid resistant strain was the only one that showed only the mutant allele. Identification of this mutation and the strong signal detected in southern blot with this strain, suggest that esterases are contributing to detoxification of pyrethroid compounds, as a resistant mechanism in Mexican strains of the southern cattle tick.
从微小牛蜱的敏感品系和抗有机磷品系中获得了两个酯酶cDNA序列。这两个序列与羧酸酯酶B具有高度同源性。一个基因在两个品系中具有相同的序列，另一个则显示出两个点突变。当推导氨基酸序列时，一个突变产生了氨基酸替换，这种突变在对杀虫剂敏感和抗性的六个不同种群中均被检测到，但仅在一个抗拟除虫菊酯品系中发现了仅携带突变等位基因的情况。这种突变的鉴定以及在该品系的Southern印迹中检测到的强信号表明，酯酶作为墨西哥南方牛蜱品系中的一种抗性机制，有助于拟除虫菊酯类化合物的解毒。
