高效液相色谱法同时测定片剂中的噻苯达唑和甲苯达唑

Simultaneous determination of thiabendazole and mebendazole in tablets by high-performance liquid chromatography.

作者信息

Orsine E M, Kedor-Hackmann E R, Santoro M I

机构信息

Faculty of Pharmaceutical Sciences, University of São Paulo, Brasil.

出版信息

Drug Dev Ind Pharm. 2000 Aug;26(8):879-83. doi: 10.1081/ddc-100101312.

DOI:10.1081/ddc-100101312

PMID:10900545

Abstract

Reversed-phase high performance liquid chromatography using an RP 18 column (4 x 125 mm), tetrahydrofuran-acetonitrile-0.5% formic acid (5:25:70, v/v/v) as mobile phase and UV detection at 254 nm enabled the simultaneous determination of thiabendazole (TZ) and mebendazole (MZ) in tablets. The method showed linearity over 4.0 to 40.0 micrograms TZ/ml and 6.0 to 60.0 micrograms MZ/ml. The correlation coefficient r was .9999 for both TZ and MZ. The coefficient of variation (CV) was 0.59-0.80% for TZ and 0.49-0.67% for MZ. The average recovery was 100.54-101.17% for TZ and 100.35-101.13% for MZ. The excipients of the tablets did not interfere in the proposed method. The developed method is precise, accurate, and selective for the determination of both benzimidazoles analyzed.

摘要

使用RP 18柱（4×125 mm）的反相高效液相色谱法，以四氢呋喃 - 乙腈 - 0.5%甲酸（5:25:70，v/v/v）作为流动相，并在254 nm处进行紫外检测，能够同时测定片剂中的噻苯达唑（TZ）和甲苯达唑（MZ）。该方法在4.0至40.0微克TZ/毫升和6.0至60.0微克MZ/毫升范围内呈线性。TZ和MZ的相关系数r均为0.9999。TZ的变异系数（CV）为0.59 - 0.80%，MZ的变异系数为0.49 - 0.67%。TZ的平均回收率为100.54 - 101.17%，MZ的平均回收率为100.35 - 101.13%。片剂中的辅料对所提出的方法没有干扰。所建立的方法对于所分析的两种苯并咪唑的测定具有精密度高、准确度高和选择性好的特点。