Sampson M L, Csako G, Remaley A T
Clinical Pathology Department, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Ann Clin Biochem. 2000 Jul;37 ( Pt 4):479-87. doi: 10.1177/000456320003700408.
The quantitation of cholesterol in lipoprotein subfractions is valuable in estimating the risk for coronary artery disease, but requires multiple tests. We describe a relatively simple procedure, referred to as the dual HDL/total cholesterol (DHT) assay, which allows the sequential measurement of HDL cholesterol (HDL-C) and total cholesterol (total-C) in a single tube. HDL-C is first measured using a homogeneous assay that utilizes an anti-apolipoprotein B [apo(B)] antibody, which sterically blocks the enzymatic measurement of cholesterol on the non-HDL subfractions. Next, deoxycholate is added, which disrupts the antibody-apo(B) complex and allows the subsequent enzymatic measurement of the remaining cholesterol in the non-HDL subfractions. The DHT assay has an acceptable analytical performance and yields results similar to standard methods: for HDL-C, y(DHT) = 0.98x + 0.19, r=0.90; for total-C, y(DHT) = 1.11x - 0.09, r=0.99. In summary, the DHT assay is a homogeneous assay for both HDL-C and total-C, and provides a simple and cost-effective method for screening for hyperlipidaemia.
脂蛋白亚组分中胆固醇的定量对于评估冠状动脉疾病风险很有价值，但需要进行多项检测。我们描述了一种相对简单的方法，称为双高密度脂蛋白/总胆固醇（DHT）测定法，该方法可在单个试管中依次测量高密度脂蛋白胆固醇（HDL-C）和总胆固醇（total-C）。首先使用一种均相测定法测量HDL-C，该方法利用抗载脂蛋白B [apo(B)]抗体，该抗体在空间上阻断非HDL亚组分上胆固醇的酶促测量。接下来，加入脱氧胆酸盐，它会破坏抗体-apo(B)复合物，并允许随后对非HDL亚组分中剩余胆固醇进行酶促测量。DHT测定法具有可接受的分析性能，其结果与标准方法相似：对于HDL-C，y(DHT)=0.98x + 0.19，r = 0.90；对于总胆固醇，y(DHT)=1.11x - 0.09，r = 0.99。总之，DHT测定法是一种用于HDL-C和总胆固醇的均相测定法，为高脂血症筛查提供了一种简单且经济高效的方法。
