Sampson M L, Aubry A, Csako G, Remaley A T
Clinical Chemistry Service, Department of Laboratory Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.
Clin Chem. 2001 Mar;47(3):532-9.
The analysis of lipids in serum lipoprotein fractions is useful in assessing the risk for coronary artery disease, but it typically involves performing multiple tests. An automated single-tube assay, referred to as the triple lipid screening (TLS) test, can be used for measuring HDL-cholesterol (HDL-C), total cholesterol, and triglycerides (TGs) with no specimen pretreatment.
The first part of the assay is based on a homogeneous assay for HDL-C that uses either an anti-apolipoprotein B antibody (TLS-A test) or a polyanion (TLS-B test) that blocks the enzymatic measurement of cholesterol on the non-HDL fraction. After the addition of deoxycholate, which solubilizes the unreacted cholesterol from the non-HDL fraction, the remaining cholesterol in the sample is subsequently measured enzymatically. Using the same enzyme detection system as the cholesterol assay, TGs are measured in the last step, after the addition of the enzymes for the TG assay.
The TLS assay (y) had acceptable analytic performance and compared favorably with standard tests (x) for each analyte: for HDL-C, TLS-A = 0.99x + 0.19 (R = 0.980); TLS-B = 1.00x - 0.15 (R = 0.974); for total cholesterol, TLS-A = 1.03x + 0.12 (R = 0.997); TLS-B = 1.07x - 0.30 (R = 0.965); and for TGs, TLS-A = 1.02x + 0.02 (R = 0.988); TLS-B = 1.04x - 0.28 (R = 0.980).
The TLS test is a single-tube homogeneous assay for the analysis of all of the major serum lipoprotein fractions and can be used as a simple screening test for the detection of hyperlipidemia.
对血清脂蛋白组分中的脂质进行分析有助于评估冠状动脉疾病风险，但通常需要进行多项检测。一种名为三联脂质筛查（TLS）检测的自动化单管检测方法，可用于在无需对样本进行预处理的情况下测量高密度脂蛋白胆固醇（HDL-C）、总胆固醇和甘油三酯（TGs）。
该检测的第一部分基于一种用于HDL-C的均相检测方法，该方法使用抗载脂蛋白B抗体（TLS-A检测）或一种多聚阴离子（TLS-B检测）来阻断非HDL组分中胆固醇的酶促测量。加入脱氧胆酸盐使非HDL组分中未反应的胆固醇溶解后，随后对样品中剩余的胆固醇进行酶促测量。使用与胆固醇检测相同的酶检测系统，在加入用于TG检测的酶后，于最后一步测量TGs。
TLS检测（y）具有可接受的分析性能，并且与每种分析物的标准检测（x）相比表现良好：对于HDL-C，TLS-A = 0.99x + 0.19（R = 0.980）；TLS-B = 1.00x - 0.15（R = 0.974）；对于总胆固醇，TLS-A = 1.03x + 0.12（R = 0.997）；TLS-B = 1.07x - 0.30（R = 0.965）；对于TGs，TLS-A = 1.02x + 0.02（R = 0.988）；TLS-B = 1.04x - 0.28（R = 0.980）。
TLS检测是一种用于分析所有主要血清脂蛋白组分的单管均相检测方法，可作为检测高脂血症的简单筛查试验。
