Zschocke S, Drewes S E, Paulus K, Bauer R, van Staden J
Research Centre for Plant Growth and Development, School of Botany and Zoology, University of Natal Pietermaritzburg, Private Bag X01, 3209, Scottsville, South Africa.
J Ethnopharmacol. 2000 Jul;71(1-2):219-30. doi: 10.1016/s0378-8741(00)00159-8.
Ocotea bullata (Lauraceae), one of the top-ten traditional medicinal plants used in KwaZulu-Natal, is close to extinction through high demand and destructive harvesting methods. The stem bark is traditionally used to cure headaches, urinary disorders and stomach problems. Substitution of leaves for bark provides a possible resource management solution for this threatened medicinal plant. One aim of this study was to compare the chemical composition of O. bullata leaves and bark using TLC, HPLC and GC-MS analysis. The characteristic analytical fingerprints of leaf and bark extracts showed great similarities. A second aim was to investigate the pharmacological properties of O. bullata as a remedy against headaches. Leaf and bark extracts were tested in terms of cyclooxygenase-1 and 5-lipoxygenase inhibition. Extracts from the bark exhibited moderate inhibitory activity in both test systems. Extracts from fresh leaves were superior to bark extracts in terms of their in vitro inhibitory activity against cyclooxygenase-1 and 5-lipoxygenase. Volatiles obtained from n-hexane extracts of leaves and bark showed better inhibitory activity towards cyclooxygenase-1 and especially towards 5-lipoxygenase than the original n-hexane extracts. Volatiles were therefore recognized as one of the main active principles in O. bullata with regards to the anti-inflammatory properties of this medicinal plant. This lends support to the traditional usage of O. bullata bark as an inhalant or snuff.
布尔塔奥寇梯木（樟科）是夸祖鲁 - 纳塔尔省使用的十大传统药用植物之一，由于需求旺盛和破坏性的采收方式，已濒临灭绝。其茎皮传统上用于治疗头痛、泌尿系统疾病和胃部问题。用树叶替代树皮为这种濒危药用植物提供了一种可能的资源管理解决方案。本研究的一个目的是使用薄层色谱法（TLC）、高效液相色谱法（HPLC）和气相色谱 - 质谱联用分析法（GC - MS）比较布尔塔奥寇梯木叶和树皮的化学成分。叶和树皮提取物的特征分析指纹图谱显示出极大的相似性。第二个目的是研究布尔塔奥寇梯木作为治疗头痛药物的药理特性。对叶和树皮提取物进行了环氧化酶 - 1（COX - 1）和5 - 脂氧合酶（5 - LOX）抑制方面的测试。树皮提取物在两个测试系统中均表现出中等抑制活性。新鲜树叶提取物在体外对环氧化酶 - 1和5 - 脂氧合酶的抑制活性方面优于树皮提取物。从叶和树皮的正己烷提取物中获得的挥发物对环氧化酶 - 1尤其是对5 - 脂氧合酶的抑制活性比原始正己烷提取物更好。因此，就这种药用植物的抗炎特性而言，挥发物被认为是布尔塔奥寇梯木的主要活性成分之一。这为布尔塔奥寇梯木树皮作为吸入剂或鼻烟的传统用法提供了支持。
