Tang L K, Spies H G
Endocrinology. 1975 Feb;96(2):349-55. doi: 10.1210/endo-96-2-349.
The effects of 17beta-estradiol (E2), progesterone (P4), 20alpha-hydroxypregn-4-en-3-one (20alpha-OHP4), and testosterone (T) on the basal and LRF-induced secretion of LH and FSH were studies in monolayer cultures prepared from the pituitaries of adult female rats. Day 4 cultures were used and all steroids were tested at 10-8M concentration for 4 hr. E2 (2.72 ng/ml) alone caused a nonsignificant increase in basal secretion of both LH and FSH; however, the same dose of E2 significantly (p less than 0.001) inhibited the LRF-induced secretion of LH but not of FSH (74% and 88% of 10-8M LRF-treated level, respectively), Testosterone (2.88 ng/ml) alone significantly increased the basal secretion of LH (136% of control level, p less than 0.05) and augmented the effect of LRF on FSH secretion to 130% of the LRF-treated level (p less than 0.05). Contrary to its negative feedback action on the basal secretion of FSH (46% of control level, P less than 0.05), 20ALPHA-OPH4 (3.15 ng/ml) augmented the effect of LRF on LH secretion (130% of LRF-treated level, P less than 0.05). On the other hand, P4 (3.14 ng/ml) did not cause any significant inhibition in the basal and LRF-induced secretion of either LH or FSH. These data indicate that both of the LH- and FSH-LRF; however, their secretory activities are modulated differently by various steroids.
在由成年雌性大鼠垂体制备的单层培养物中，研究了17β-雌二醇（E2）、孕酮（P4）、20α-羟基孕-4-烯-3-酮（20α-OHP4）和睾酮（T）对促黄体生成素（LH）和促卵泡生成素（FSH）基础分泌及促性腺激素释放因子（LRF）诱导分泌的影响。使用第4天的培养物，所有类固醇均以10-8M浓度测试4小时。单独使用E2（2.72 ng/ml）使LH和FSH的基础分泌有不显著增加；然而，相同剂量的E2显著（p小于0.001）抑制LRF诱导的LH分泌，但不抑制FSH分泌（分别为10-8M LRF处理水平的74%和88%）。单独使用睾酮（2.88 ng/ml）显著增加LH的基础分泌（为对照水平的136%，p小于0.05），并将LRF对FSH分泌的作用增强至LRF处理水平的130%（p小于0.05）。与它对FSH基础分泌的负反馈作用相反（为对照水平的46%，P小于0.05），20α-OHP4（3.15 ng/ml）增强了LRF对LH分泌的作用（为LRF处理水平的130%，P小于0.05）。另一方面，P4（3.14 ng/ml）对LH或FSH的基础分泌及LRF诱导的分泌均未引起任何显著抑制。这些数据表明，LH和FSH对LRF均有反应；然而，它们的分泌活动受到各种类固醇的不同调节。
