Myers L L
Infect Immun. 1975 Mar;11(3):493-6. doi: 10.1128/iai.11.3.493-496.1975.
A total of 373 isolates of Escherichia coli were obtained from one or more calves with diarrhea in 155 herds during the 1974 calving season in Montana. Sixty-seven (18 percent) of the isolates representing 59 of the 155 herds were found to be enterotoxigenic as indicated by their ability to cause distention of the calf ligated intestinal segment. The 67 isolates of enterotoxigenic E. coli (ETEC) were placed in one of six different antigenic groups based upon agglutination of formolized whole cells in KO antiserum. Eighty-seven percent (58 of 67) of the ETEC had antigen 1, 2, or 3, whereas only 2.3 per cent (7 of 310) of the non-ETEC (NETEC) had antigen 1, 2, or 3. This antigen numbering system was used for convenience and is not related to any established typing system. Antigens 1, 2, and 3 do not belong to any of the O groups 1 to 157 or K groups 1 to 93 of the International Schema. Colony color or morphology of ETEC and NETEC grown on Tergitol-7 agar with triphenyltetrazolium chloride added could not be used as an indicator of enterotoxigenicity, although there was a tendency for E. coli with the smooth and mucoid colony type to be enterotoxigenic whereas rough colonies were seldom enterotoxigenic. Among ETEC isolates with antigen 1, 2, or 3, there was good correlation between colony type and antigen number. All 13 ETEC isolates with antigen 1 had the smooth colony type, 17 of 19 ETEC isolates with antigen 2 had the smooth, mucoid colony type, and all 25 isolates of ETEC with antigen 3 had the intermediate colony type. Conversely, of the 310 isolates of NETEC, none had antigen 1 and the smooth colony type; none had antigen 2 and the smooth and mucoid colony type; and only one isolate of NETEC had antigen 3 and the intermediate colony type. Sixteen of the 67 isolates of ETEC (24 percent) were motile. Fifteen of the 16 motile isolates of ETEC had the intermediate colony type, and none of the ETEC with smooth or smooth and mucoid colonies were motile.
1974年蒙大拿州产犊季节期间，从155个牛群中一个或多个腹泻犊牛身上共获得373株大肠杆菌分离株。其中67株（占18%）代表155个牛群中的59个，经结扎犊牛肠段试验表明具有致肠段扩张能力，即被认定为产肠毒素性大肠杆菌（ETEC）。根据福尔马林处理的全细胞在KO抗血清中的凝集情况，将这67株产肠毒素性大肠杆菌分为六个不同抗原组中的一组。87%（67株中的58株）的产肠毒素性大肠杆菌具有抗原1、2或3，而非产肠毒素性大肠杆菌（NETEC）中只有2.3%（310株中的7株）具有抗原1、2或3。这种抗原编号系统只是为了方便使用，与任何既定的分型系统无关。抗原1、2和3不属于国际分型方案中的O组1至157或K组1至93。在添加了氯化三苯基四氮唑的Tergitol - 7琼脂上生长的产肠毒素性大肠杆菌和非产肠毒素性大肠杆菌的菌落颜色或形态不能作为产肠毒素性的指标，不过，光滑和黏液样菌落类型的大肠杆菌有产肠毒素的倾向，而粗糙菌落很少产肠毒素。在具有抗原1、2或3的产肠毒素性大肠杆菌分离株中，菌落类型与抗原编号之间存在良好的相关性。所有13株具有抗原1的产肠毒素性大肠杆菌分离株均为光滑菌落类型，19株具有抗原2的产肠毒素性大肠杆菌分离株中有17株为光滑、黏液样菌落类型，所有25株具有抗原3的产肠毒素性大肠杆菌分离株均为中间型菌落类型。相反，在310株非产肠毒素性大肠杆菌分离株中，没有一株具有抗原1和光滑菌落类型；没有一株具有抗原2和光滑及黏液样菌落类型；只有一株非产肠毒素性大肠杆菌分离株具有抗原3和中间型菌落类型。67株产肠毒素性大肠杆菌分离株中有16株（24%）具有运动性。16株具有运动性的产肠毒素性大肠杆菌分离株中有15株为中间型菌落类型，具有光滑或光滑和黏液样菌落的产肠毒素性大肠杆菌均无运动性。
