Scherer R K
Appl Microbiol. 1966 Mar;14(2):152-5. doi: 10.1128/am.14.2.152-155.1966.
Escherichia coli cultures grown on Tergitol-7 medium, with 2,3,5-triphenyltetrazolium chloride added, produced three main types of colonies: rough, intermediate, and mucoid. These colonies were yellow to amber in color and produced slight yellow zones in the medium. Rough colonies were flat, dry, and spreading, with a cut-glass appearance. Intermediate-type colonies varied considerably, but could be divided into two general subtypes. Intermediate-rough colonies had the cut-glass appearance characteristic of rough colonies, but were much more compact and raised, with irregular edges. Intermediate-smooth colonies had a slight cut-glass appearance, but were smooth and entire. Mucoid-type colonies also appeared in two subtypes. Mucoid A colonies were mucoid hemispheres. Mucoid B colonies, after incubation at 37 C for 24 hr, appeared as small, intermediate colonies. However, during a 24-hr holding period at room temperature, mucuslike material proliferated around the colonies. A fourth type of colony was red with blue surrounding medium. Only mucoid-type cultures could not be serologically O-grouped.
在添加了氯化三苯基四氮唑的特基托-7培养基上培养的大肠杆菌产生了三种主要类型的菌落：粗糙型、中间型和黏液型。这些菌落颜色从黄色到琥珀色不等，并且在培养基中产生浅黄色区域。粗糙型菌落扁平、干燥且蔓延生长，具有磨砂玻璃外观。中间型菌落差异很大，但可分为两种一般亚型。中间粗糙型菌落具有粗糙型菌落特有的磨砂玻璃外观，但更加紧凑且凸起，边缘不规则。中间光滑型菌落有轻微的磨砂玻璃外观，但表面光滑且完整。黏液型菌落也有两种亚型。黏液A型菌落是黏液半球形。黏液B型菌落在37℃孵育24小时后，呈现为小的中间型菌落。然而，在室温下放置24小时期间，菌落周围会增殖出黏液状物质。第四种类型的菌落呈红色，周围培养基呈蓝色。只有黏液型培养物无法进行血清学O群分类。
