Kamal-Eldi A, Görgen S, Pettersson J, Lampi A M
Department of Food Science, Swedish University of Agricultural Sciences, Uppsala.
J Chromatogr A. 2000 Jun 9;881(1-2):217-27. doi: 10.1016/s0021-9673(99)01346-1.
Natural vitamin E is composed of eight different vitamers (alpha-, beta-, gamma- and delta-tocopherols and alpha-, beta-, gamma- and delta-tocotrienols). As these eight vitamers have different antioxidant and biological activities, it is necessary to have quantitative data on each substance separately. The aim of this study was to find universal HPLC columns for the separation of all eight components and to test if a few columns of the same material (different batches) will give reproducible results. Normal-phase HPLC separations of vitamin E compounds in a prepared mixture (containing oat extracts, palm oil and tocopherol standards) were tried on six silica, three amino and one diol columns. As shown by calculations of retention factors (k), separation factors (alpha), numbers of theoretical plates (N) and resolutions (Rs), the best separations were obtained on three silica columns and two amino columns using 4 or 5% dioxane in hexane as the mobile phase as well as on a diol column using 4% tert.-butyl methyl ether in hexane as the mobile phase.
天然维生素E由八种不同的维生素形式（α-、β-、γ-和δ-生育酚以及α-、β-、γ-和δ-生育三烯酚）组成。由于这八种维生素形式具有不同的抗氧化和生物活性，因此有必要分别获取每种物质的定量数据。本研究的目的是找到用于分离所有八种成分的通用高效液相色谱（HPLC）柱，并测试同一材料的几根柱（不同批次）是否能给出可重复的结果。在六个硅胶柱、三个氨基柱和一个二醇柱上尝试了对制备好的混合物（含燕麦提取物、棕榈油和生育酚标准品）中的维生素E化合物进行正相HPLC分离。通过保留因子（k）、分离因子（α）、理论塔板数（N）和分离度（Rs）的计算表明，使用己烷中4%或5%的二氧六环作为流动相时，在三根硅胶柱和两根氨基柱上以及使用己烷中4%的叔丁基甲基醚作为流动相时在一根二醇柱上获得了最佳分离效果。
