Yoshida N
J Protozool. 1975 Feb;22(1):128-30. doi: 10.1111/j.1550-7408.1975.tb00957.x.
A macromolecule-free medium, containing in its defined part 3 salts, glucose, hemin, 21 amino acids, 3 lipids, and some undefined components obtained by dialysis of liver infusion, was developed for serial cultivation of Trypanosoma cruzi at 28 C. The medium allows prolonged cultivation of T. cruzi by serial transfers and growth comparable to that obtained in more complex media, including those containing blood serum.
