O'Daly J A
J Protozool. 1975 May;22(2):265-70. doi: 10.1111/j.1550-7408.1975.tb05864.x.
A new liquid medium, with fetal calf serum as the sole undefined component, was devised for the cultivation of Trypanosoma cruzi. The need for the serum is ascribed to its mitogenic proteins, which stimulate division of, and the uptake of [3-H-] thymidine by the parasites. In the new medium, T. cruzi has a cycle culminating in the apperance of up to 90 per cent metacyclic forms in the stationary phase. This cycle is repeated on each serial transfer.
一种以胎牛血清作为唯一未定义成分的新型液体培养基被设计用于培养克氏锥虫。对血清的需求归因于其促有丝分裂蛋白，这些蛋白可刺激寄生虫的分裂以及[3-H-]胸腺嘧啶核苷的摄取。在这种新培养基中，克氏锥虫具有一个周期，在稳定期可产生高达90%的循环后期形式。每次传代时这个周期都会重复。
