O'Daly J A, Rodríguez M B, Garlin G
Exp Parasitol. 1987 Aug;64(1):78-87. doi: 10.1016/0014-4894(87)90011-7.
An enriched synthetic medium with low molecular weight peptides allows Trypanosoma cruzi epimastigotes to grow at 26-37 C. Using this medium, the growth requirements of T. cruzi were compared at different temperatures. When supplemented with fetal bovine serum or serum peptides, nine amino acids were absolutely required from the first passage, while additional amino acids and amino acid precursors were needed to support growth during a second passage. Five amino acids (beta-alanine, glutamine, cysteine, ornithine, and threonine) were also required absolutely at temperatures ranging between 30 and 37 C. Nine vitamins were needed at all temperatures, while ascorbic acid and ergocalciferol were not necessary at any temperature. The remaining amino acids and vitamins showed a variable role as growth factors depending on the temperature increase. In peptide supplemented media, requirements for amino acids and their precursors, as well as vitamins and nucleotides, increased markedly when compared with the protein supplemented medium. A peptide composed of one glutamic acid, two alanines, and one lysine can substitute for serum for trypanosomal growth at all temperatures. Several minimum media have been prepared in which epimastigote forms of T. cruzi can grow at 26-37 C for more than 10 passages.
一种含有低分子量肽的富集合成培养基能使克氏锥虫前鞭毛体在26至37摄氏度下生长。利用这种培养基，在不同温度下比较了克氏锥虫的生长需求。当添加胎牛血清或血清肽时，从第一代传代起就绝对需要9种氨基酸，而在第二代传代期间则需要额外的氨基酸和氨基酸前体来支持生长。在30至37摄氏度的温度范围内也绝对需要5种氨基酸（β-丙氨酸、谷氨酰胺、半胱氨酸、鸟氨酸和苏氨酸）。在所有温度下都需要9种维生素，而抗坏血酸和麦角钙化醇在任何温度下都不需要。其余的氨基酸和维生素作为生长因子的作用随温度升高而变化。在添加肽的培养基中，与添加蛋白质的培养基相比，对氨基酸及其前体以及维生素和核苷酸的需求显著增加。一种由一个谷氨酸、两个丙氨酸和一个赖氨酸组成的肽在所有温度下都能替代血清用于锥虫生长。已经制备了几种基本培养基，在其中克氏锥虫前鞭毛体形式能在26至37摄氏度下生长超过10代。
