Ku S K, Lee H S, Lee J H
Central Research Laboratories, Dong-Wha Pharm. Ind. Co., Anyang, Korea.
J Vet Med Sci. 2000 Jun;62(6):589-94. doi: 10.1292/jvms.62.589.
The regional distribution and relative frequency of endocrine cells was studied immunohistochemically (PAP method) in the alimentary tract of the red-bellied frog, Bombina orientalis, using antisera against serotonin, somatostatin, chromogranin (CG), cholecystokinin (CCK)-8, bombesin, secretin, glucagon and pancreatic polypeptide (PP). Eight kinds of endocrine cells were identified in this study. These immunoreactive cells were located in the gastric glands of the stomach regions and in the intestinal or esophageal epithelium with variable frequencies. They were spherical or spindle-shaped. Serotonin- and somatostatin-immunoreactive cells were demonstrated in the whole alimentary tract including esophagus. CG-immunoreactive cells were restricted to the stomach. CCK-8-immunoreactive cells were observed from the antrum to the ileum. Bombesin-immunoreactive cells were restricted to the stomach. Secretin-immunoreactive cells were demonstrated in the pylorus, duodenum and ileum. Glucagon-immunoreactive cells were found in the antrum and duodenum. PP-immunoreactive cells were detected from the antrum to the rectum. In conclusion, throughout the alimentary tract of the red-bellied frog, the different regional distribution and relative frequency of endocrine cells were demonstrated. The regional distributions and relative frequencies of the endocrine cells in the alimentary tract of the red-bellied frog were resembled to those of the other anuran species except for esophagus.
采用抗血清检测5-羟色胺、生长抑素、嗜铬粒蛋白（CG）、胆囊收缩素（CCK）-8、蛙皮素、促胰液素、胰高血糖素和胰多肽（PP），运用免疫组织化学方法（PAP法）研究了东方铃蟾消化道内分泌细胞的区域分布及相对频率。本研究鉴定出8种内分泌细胞。这些免疫反应性细胞以不同频率分布于胃区的胃腺以及肠或食管上皮中。它们呈球形或梭形。5-羟色胺和生长抑素免疫反应性细胞在包括食管在内的整个消化道中均有显示。CG免疫反应性细胞局限于胃。CCK-8免疫反应性细胞在胃窦至回肠均有观察到。蛙皮素免疫反应性细胞局限于胃。促胰液素免疫反应性细胞在幽门、十二指肠和回肠中有显示。胰高血糖素免疫反应性细胞在胃窦和十二指肠中发现。PP免疫反应性细胞在胃窦至直肠均有检测到。总之，在东方铃蟾的整个消化道中，显示出内分泌细胞不同的区域分布及相对频率。除食管外，东方铃蟾消化道内分泌细胞的区域分布和相对频率与其他无尾类物种相似。
