Lee H S, Choi S H, Ku S K
Department of Clinical Laboratory Science, College of Health and Therapy, Daegu Haany University, Gyeongsan, 712-715, Republic of Korea.
Anat Histol Embryol. 2010 Dec;39(6):521-8. doi: 10.1111/j.1439-0264.2010.01024.x. Epub 2010 Aug 15.
The distributions and frequencies of some endocrine cells in the eight portions of the gastrointestinal (GI) tract - fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum of the ddN mouse, were studied with immunohistochemical method using seven types of antisera against chromogranin (Cg) A serotonin, somatostatin, glucagon, gastrin, cholecystokinin (CCK)-8 and human pancreatic polypeptide (hPP). In the GI tract of ddN mice, CgA, serotonin, somatostatin, glucagon, gastrin, CCK-8 immunoreactive (IR) cells were identified with various frequencies, but hPP-IR cells were not observed in this study. Most of IR cells in the intestinal portion were generally spherical or spindle in shape (open type cell) whereas cells showing round in shape (close type cell) were found in the intestinal gland and stomach regions occasionally. They showed the highest frequency in the pylorus or colon. CgA-IR cells were observed from the pylorus to ileum. Serotonin-IR cells were detected throughout the whole GI tract except for the fundus. Somatostatin-IR cells were demonstrated throughout the whole GI tract except for the cecum and colon. Gastrin and CCK-8-IR cells were restricted to the pylorus and duodenum. In addition, a few glucagon-IR cells were restricted to the fundus and rectum. In conclusion, the general distribution patterns and relative frequency of GI endocrine cells of the ddN mouse was similar to that of other strains of mice. However, some strain and/or species-dependent unique distributions and frequencies of endocrine cells were also observed in the present study.
采用七种抗血清，即抗嗜铬粒蛋白（Cg）A、5-羟色胺、生长抑素、胰高血糖素、胃泌素、胆囊收缩素（CCK）-8和人胰多肽（hPP）的抗血清，运用免疫组织化学方法，研究了ddN小鼠胃肠道八个部位（胃底、幽门、十二指肠、空肠、回肠、盲肠、结肠和直肠）中一些内分泌细胞的分布及频率。在ddN小鼠的胃肠道中，已鉴定出不同频率的CgA、5-羟色胺、生长抑素、胰高血糖素、胃泌素、CCK-8免疫反应（IR）细胞，但在本研究中未观察到hPP-IR细胞。肠道部分的大多数IR细胞通常呈球形或梭形（开放型细胞），而偶尔在肠腺和胃区域发现呈圆形的细胞（封闭型细胞）。它们在幽门或结肠中的频率最高。从幽门到回肠均观察到CgA-IR细胞。除胃底外，在整个胃肠道均检测到5-羟色胺-IR细胞。除盲肠和结肠外，在整个胃肠道均证实有生长抑素-IR细胞。胃泌素和CCK-8-IR细胞局限于幽门和十二指肠。此外，少数胰高血糖素-IR细胞局限于胃底和直肠。总之，ddN小鼠胃肠道内分泌细胞的总体分布模式和相对频率与其他品系小鼠相似。然而，在本研究中也观察到了一些品系和/或物种依赖性的内分泌细胞独特分布和频率。
