Ku S K, Lee H S, Lee J H, Park K D
Department of Biology, Faculty of Basic Science, Kyungsan University, Kyungsan, Republic of Korea.
Anat Histol Embryol. 2001 Feb;30(1):33-9. doi: 10.1046/j.1439-0264.2001.00284.x.
The regional distribution and relative frequency of endocrine cells in the alimentary tract of the red-eared slider, Trachemys scripta elegans, were investigated by immunohistochemical methods using 10 antisera. Most of the immunoreactive cells in the intestine were spherical or spindle-like in shape (open-type cells), while round cells (closed-type cells) were occasionally found in the stomach. These immunoreactive cells were located in the basal portion of the intestine, including the oesophagus, and in the gastric glands of the stomach. Cg A-immunoreactive cells were restricted to the pylorus and duodenum and were few in number. Serotonin-immunoreactive cells, which were most commonly found in the pylorus, were found in the epithelia throughout the alimentary tract at various frequencies. Gastrin-immunoreactive cells were found in the pylorus, duodenum and jejunum at moderate, low and very low frequencies, respectively. Somatostatin-immunoreactive cells were found throughout the alimentary tract except for the rectum, at various frequencies. Glucagon-immunoreactive cells were detected in the fundus, pylorus, jejunum and ileum at low or very low frequencies. CCK-8-immunoreactive cells were found in the pylorus, fundus and duodenum at very low, low and moderate frequencies, respectively. Bombesin-immunoreactive cells were restricted to the fundus and pylorus at low frequencies. No secretin-, BPP- or VIP-immunoreactive cells were found in this study.
采用10种抗血清，通过免疫组织化学方法研究了红耳龟（Trachemys scripta elegans）消化道内分泌细胞的区域分布和相对频率。肠道中大多数免疫反应性细胞呈球形或纺锤形（开放型细胞），而圆形细胞（封闭型细胞）偶尔见于胃中。这些免疫反应性细胞位于包括食管在内的肠道基部以及胃的胃腺中。嗜铬粒蛋白A免疫反应性细胞局限于幽门和十二指肠，数量较少。5-羟色胺免疫反应性细胞最常见于幽门，在整个消化道上皮中均有不同频率的分布。胃泌素免疫反应性细胞分别以中等、低和极低频率见于幽门、十二指肠和空肠。生长抑素免疫反应性细胞在除直肠外的整个消化道中均有不同频率的分布。胰高血糖素免疫反应性细胞在胃底、幽门、空肠和回肠中以低或极低频率被检测到。胆囊收缩素-8免疫反应性细胞分别以极低、低和中等频率见于幽门、胃底和十二指肠。蛙皮素免疫反应性细胞局限于胃底和幽门，频率较低。本研究未发现促胰液素、胰多肽或血管活性肠肽免疫反应性细胞。
