Yamauchi K, Hamasaki S, Miyazaki K, Kikusui T, Takeuchi Y, Mori Y
Laboratory of Veterinary Ethology, The University of Tokyo, Japan.
J Vet Med Sci. 2000 Jun;62(6):669-71. doi: 10.1292/jvms.62.669.
A sex determination method using DNA extracted from feces has been developed for sika deer (Cervus nippon). We determined a partial sequence of the amelogenin gene of sika deer, which exists on both X and Y chromosomes with a deletion region on the Y chromosome. Based on the sexually dimorphic sequences, we designed a pair of primers which could amplify DNA fragments the lengths of which are different between males and females. PCR products were detected in 34 out of 37 fecal samples collected from captured deer and the sexes estimated by the present method were perfectly matched with the actual sexes.
已开发出一种利用从粪便中提取的DNA来鉴定梅花鹿（Cervus nippon）性别的方法。我们测定了梅花鹿牙釉蛋白基因的部分序列，该基因存在于X和Y染色体上，Y染色体上有一个缺失区域。基于两性异形序列，我们设计了一对引物，该引物能够扩增出雄性和雌性长度不同的DNA片段。在从捕获的鹿身上采集的37份粪便样本中，有34份检测到了PCR产物，通过本方法估算出的性别与实际性别完全匹配。
