人类专用PowerPlex 21系统（美国普洛麦格公司）在黑猩猩（黑猩猩属）中的功能验证。
Functional validation of human-specific PowerPlex 21 System (Promega, USA) in chimpanzee (Pan troglodytes).
作者信息
Thakur Mukesh, Chandra Kailash, Sahajpal Vivek, Samanta Asis, Sharma Arun, Mitra Agni
机构信息
Zoological Survey of India, New Alipore, Kolkata, West Bengal, 700053, India.
State Forensic Science Laboratory, Junga, Shimla, Himachal Pradesh, 171218, India.
出版信息
BMC Res Notes. 2018 Oct 3;11(1):695. doi: 10.1186/s13104-018-3803-x.
OBJECTIVE
This study was aimed to test the PowerPlex 21 System (Promega, USA), used for human identification applications for its positive cross-species applicability in Chimpanzees (Pan troglodytes) in order to identify heterologous STRs which can be used for individual identification, paternity testing, relatedness establishment and reconstruction of pedigrees and studbook records for captive and wild chimpanzee breeding populations.
RESULTS
Of 21 STRs in PowerPlex 21 System (Promega, USA), 19 loci amplified and found to be polymorphic. Locus Aml showed differential banding patterns in males and females similar to those seen for humans and correctly assigned sexes of known identity. Altogether, 58 different alleles were found with an average 3.05 ± 0.28 alleles per locus. Mean observed (Ho), and expected heterozygosity (He) were 0.93 ± 0.03 and 0.52 ± 0.05, respectively.
目的
本研究旨在测试用于人类身份识别的PowerPlex 21系统（美国Promega公司），以检验其在黑猩猩（Pan troglodytes）中是否具有阳性跨物种适用性，从而鉴定可用于个体识别、亲子鉴定、亲缘关系确定以及圈养和野生黑猩猩繁殖种群系谱和种畜登记簿记录重建的异源短串联重复序列（STR）。
结果
在PowerPlex 21系统（美国Promega公司）的21个STR中，19个位点扩增成功并发现具有多态性。Aml位点在雄性和雌性中显示出与人类相似的差异条带模式，并正确地确定了已知身份个体的性别。总共发现了58个不同的等位基因，每个位点平均有3.05±0.28个等位基因。观察到的平均杂合度（Ho）和预期杂合度（He）分别为0.93±0.03和0.52±0.05。
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