Peumans W J, Hause B, Van Damme E J
Laboratory for Phytopathology, Katholieke Universiteit Leuven, Willen de Croylaan 42, 3001 Leuven, Belgium.
FEBS Lett. 2000 Jul 21;477(3):186-92. doi: 10.1016/s0014-5793(00)01801-9.
A galactose-specific and a mannose-specific lectin of the family of the jacalin-related lectins have been localized by immunofluorescence microscopy. The present localization studies provide for the first time unambiguous evidence for the cytoplasmic location of the mannose-specific jacalin-related lectin from rhizomes of Calystegia sepium, which definitely differs from the vacuolar location of the galactose-specific jacalin from Artocarpus integrifolia. These observations support the hypothesis that the galactose-specific jacalin-related lectins evolved from their mannose-specific homologues through the acquisition of vacuolar targeting sequences.
通过免疫荧光显微镜定位了jacalin相关凝集素家族中的一种半乳糖特异性凝集素和一种甘露糖特异性凝集素。目前的定位研究首次为来自裂叶牵牛根茎的甘露糖特异性jacalin相关凝集素的细胞质定位提供了明确证据，这与来自波罗蜜的半乳糖特异性jacalin的液泡定位明显不同。这些观察结果支持了这样一种假说，即半乳糖特异性jacalin相关凝集素是通过获得液泡靶向序列从其甘露糖特异性同源物进化而来的。
