Yasugi S, Nakamura H
Department of Biological Sciences, Graduate School of Science, Tokyo Metropolitan University, Hachioji, Japan.
Dev Growth Differ. 2000 Jun;42(3):195-7. doi: 10.1046/j.1440-169x.2000.00500.x.
Chicken embryos have been used as a model animal in developmental biology since the time of comparative and experimental embryology. Recent application of gene transfer techniques to the chicken embryo increases their value as an experimental animal. Today, gene transfer into chicken cells is performed by three major systems, lipofection, electroporation and the virus-mediated method. Each system has its own features and applicability. In this overview and the associated four minireviews, the methods and application of each system will be presented.
