Nakamura H, Watanabe Y, Funahashi J
Department of Molecular Neurobiology, Institute of Development, Aging and Cancer, Tohoku University, Sendai, Japan.
Dev Growth Differ. 2000 Jun;42(3):199-201. doi: 10.1046/j.1440-169x.2000.00501.x.
Transfection to living chick embryos in ovo by electroporation has been recently developed. In this mini-review, misexpression in brain vesicles is introduced. To transfect, expression plasmid is inserted in the brain vesicle, and the square pulse of 25 V, 50 ms was charged five times. The translation product of the transfected gene is detected 2 h after electroporation, and reaches the peak at 24 h after electroporation. Transfection is so effective that this method is contributing greatly to the study of the molecular mechanisms of morphogenesis.
最近开发了通过电穿孔将基因导入鸡胚活体的技术。在这篇小型综述中，介绍了在脑泡中的错误表达。为了进行转染，将表达质粒插入脑泡，并施加25V、50ms的方波脉冲，重复5次。转染后2小时可检测到转染基因的翻译产物，并在转染后24小时达到峰值。转染非常有效，该方法对形态发生分子机制的研究有很大贡献。
