用重组人白血病抑制因子对人冷冻胚胎进行长期培养。
Prolonged culture of human cryopreserved embryos with recombinant human leukemia inhibitory factor.
作者信息
Hsieh Y Y, Tsai H D, Chang C C, Hsu L W, Chang S C, Lo H Y
机构信息
Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan.
出版信息
J Assist Reprod Genet. 2000 Mar;17(3):131-4. doi: 10.1023/a:1009426303742.
Abstract
PURPOSE
To evaluate the efficiency of recombinant human leukemia inhibitory factor (LIF) in the prolonged culture of human cryopreserved-thawing embryos.
METHODS
After thawing, all embryos were divided into four groups: (1) Human tubal fluid (HTF), (2) HTF + LIF, (3) M3TH medium, and (4) M3TH medium plus LIF. Following prolonged culture, embryo development in each group was compared.
RESULTS
In embryo development from about the 2- to 4-cell to 9- to 16-cell stage, there were nonsignificant differences between each group. There was lower morula formation rate in group 1 (6.9%) than those in other groups (23.2%, 19.7%, 23.1%). The lower blastocyst formation in group 1 and 3 (0%, 0%) than those in group 2 and 4 (11.0%, 12.8%) were noted.
CONCLUSIONS
LIF is beneficial for preimplantation embryos. LIF does not influence the early embryo development. LIF-supplemented HTF provided a similar culture environment for thawing embryos as LIF-supplemented M3TH medium.
摘要
目的
评估重组人白血病抑制因子（LIF）在人冷冻解冻胚胎延长培养中的效率。
方法
解冻后，所有胚胎分为四组：（1）人输卵管液（HTF），（2）HTF + LIF，（3）M3TH培养基，（4）M3TH培养基加LIF。延长培养后，比较每组胚胎的发育情况。
结果
在胚胎从约2至4细胞发育到9至16细胞阶段的过程中，各组之间无显著差异。第1组的桑葚胚形成率（6.9%）低于其他组（23.2%、19.7%、23.1%）。第1组和第3组的囊胚形成率（0%、0%）低于第2组和第4组（11.0%、12.8%）。
结论
LIF对植入前胚胎有益。LIF不影响早期胚胎发育。添加LIF的HTF为解冻胚胎提供了与添加LIF的M3TH培养基相似的培养环境。
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